Validation Data Gallery
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Tested Applications
| Positive WB detected in | RAW 264.7 cells, C2C12 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
31699-1-AP targets IRX1 in WB, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | IRX1 fusion protein Ag36597 相同性解析による交差性が予測される生物種 |
| Full Name | iroquois homeobox 1 |
| Observed molecular weight | 50 kDa |
| GenBank accession number | BC166635 |
| Gene Symbol | IRX1 |
| Gene ID (NCBI) | 79192 |
| RRID | AB_3670085 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | P78414 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Iroquois homeobox protein 1 (IRX1) is a member of the Iroquois homeobox family of transcription factors which plays a crucial role in embryonic development. IRX1 has been previously shown to function as a potential tumor suppressor in rheumatoid arthritis (RA), congenital heart disease (CHD), and tumors, such as gastric cancer (GC) and osteosarcoma (PMID: 25822025, PMID: 31640472). The molecular weight of IRX1 is 50 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for IRX1 antibody 31699-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |