Validation Data Gallery
Tested Applications
| Positive WB detected in | A549 cells, A431 cells, HEK-293 cells, HepG2 cells |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
82730-10-RR targets IRS1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | IRS1 fusion protein Ag11714 相同性解析による交差性が予測される生物種 |
| Full Name | insulin receptor substrate 1 |
| Calculated molecular weight | 1242 aa, 132 kDa |
| Observed molecular weight | 160-185 kDa |
| GenBank accession number | BC053895 |
| Gene Symbol | IRS1 |
| Gene ID (NCBI) | 3667 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P35568 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Ins receptor substrate 1 (IRS1) was the first cloned and characterized member of the IRS family which are involved in ins receptor (IR) and ins-like growth factor I receptor (IGF-IR) signaling. IRS1 is phosphorylated by ins receptor tyrosine kinase and is involved in various cellular processes including DNA repair fidelity, transcriptional activity, and cell growth can support tumor development and progression. Mutations in this gene are associated with type II diabetes and susceptibility to ins resistance. IRS1 has a predicted molecular weight of 132 kDa, however, as a result of its extensive serine phosphorylation it separates on a SDS gel as a band of approximately 160-185 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for IRS1 antibody 82730-10-RR | Download protocol |
| IF protocol for IRS1 antibody 82730-10-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |