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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IRG1 Polyclonal antibody

IRG1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 28436-1-AP

Synonyms

ACOD1, CAD, IRG1

Validation Data Gallery

Applications:
  • WB analysis using 28436-1-AP

    WB analysis using 28436-1-AP

    IFN gamma+LPS treated THP-1 cells were subjected to SDS PAGE followed by western blot with 28436-1-AP (IRG1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis using 28436-1-AP

    WB analysis using 28436-1-AP

    LPS treated RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 28436-1-AP (IRG1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inIFN gamma+LPS treated THP-1 cells, LPS treated RAW 264.7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Product Information

28436-1-AP targets IRG1 in WB, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IRG1 fusion protein Ag28913 相同性解析による交差性が予測される生物種
Full Name immunoresponsive 1 homolog (mouse)
Calculated molecular weight 53 kDa
Observed molecular weight50 kDa
GenBank accession numberNM_001258406
Gene symbol IRG1
Gene ID (NCBI) 730249
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The host Immune-Responsive Gene 1 (IRG1; also called Acod1) is a mitochondrial enzyme induced under inflammatory conditions that produces the metabolite itaconate by decarboxylating cis-aconitate, a TCA cycle intermediate. Gene expression profiling studies of murine macrophages and microglial cells have revealed that IRG1 is highly expressed under pro-inflammatory conditions. Furthermore, IRG1 is highly expressed in the pregnant uterus during the early events leading to implantation, the specific phase of pregnancy in which high levels of inflammatory cytokines are secreted. IRG1 localizes to the mitochondria and may represent a key link between immunological and metabolic processes. IRG1 has crucial functions in embryonic implantation and neurodegeneration. Also, IRG1 promotes endotoxin tolerance by increasing A20 expression in macrophages via increased ROS production. (PMID: 25640654)

Protocols

Product Specific Protocols
WB protocol for IRG1 antibody 28436-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols