• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Use Able AI chat for product recommendations

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using IRF5 Polyclonal antibody (10547-1-AP)

    WB analysis using 10547-1-AP (same clone as 10547-1-PBS)

    THP-1 cells were subjected to SDS PAGE followed by western blot with 10547-1-AP (IRF5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10547-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using IRF5 Polyclonal antibody (10547-1-AP)

    WB analysis of Jurkat using 10547-1-AP (same clone as 10547-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 10547-1-AP (IRF5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10547-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using IRF5 Polyclonal antibody (10547-1-AP)

    WB analysis of Jurkat using 10547-1-AP (same clone as 10547-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 10547-1-AP (IRF5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10547-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of SH-SY5Y cells using IRF5 Polyclonal antibody (10547-1-AP)

    WB analysis of SH-SY5Y using 10547-1-AP (same clone as 10547-1-PBS)

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 10547-1-AP (IRF5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10547-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using IRF5 Polyclonal antibody (10547-1-AP)

    IP experiment of HeLa using 10547-1-AP (same clone as 10547-1-PBS)

    IP result of anti-IRF5 (IP:10547-1-AP, 4ug; Detection:10547-1-AP 1:300) with HeLa cells lysate 1600ug. This data was developed using the same antibody clone with 10547-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using IRF5 Polyclonal antibody (10547-1-AP)

    IP experiment of HeLa using 10547-1-AP (same clone as 10547-1-PBS)

    IP result of anti-IRF5 (IP:10547-1-AP, 4ug; Detection:10547-1-AP 1:300) with HeLa cells lysate 1600ug. This data was developed using the same antibody clone with 10547-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using IRF5 Polyclonal antibody (10547-1-AP)

    IHC staining of human prostate cancer using 10547-1-AP (same clone as 10547-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10547-1-AP (IRF5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10547-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using IRF5 Polyclonal antibody (10547-1-AP)

    IHC staining of human breast cancer using 10547-1-AP (same clone as 10547-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 10547-1-AP (IRF5 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10547-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using IRF5 Polyclonal antibody (10547-1-AP)

    IHC staining of human breast cancer using 10547-1-AP (same clone as 10547-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10547-1-AP (IRF5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10547-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using IRF5 Polyclonal antibody (10547-1-AP)

    IHC staining of human breast cancer using 10547-1-AP (same clone as 10547-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10547-1-AP (IRF5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10547-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using IRF5 Polyclonal antibody (10547-1-AP)

    IHC staining of human prostate cancer using 10547-1-AP (same clone as 10547-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10547-1-AP (IRF5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10547-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using IRF5 Polyclonal antibody (10547-1-AP)

    IF Staining of HepG2 using 10547-1-AP (same clone as 10547-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using IRF5 antibody (10547-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10547-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using IRF5 Polyclonal antibody (10547-1-AP)

    IF Staining of HepG2 using 10547-1-AP (same clone as 10547-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using IRF5 antibody (10547-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 10547-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using IRF5 Polyclonal antibody (10547-1-AP)

    IF Staining of HepG2 using 10547-1-AP (same clone as 10547-1-PBS)

    Immunofluorescent analysis of HepG2 cells using 10547-1-AP (IRF5 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10547-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of THP-1 cells using IRF5 Polyclonal antibody (10547-1-AP)

    FC experiment of THP-1 using 10547-1-AP (same clone as 10547-1-PBS)

    1x10^6 THP-1 cells were intracellularly stained with 0.25 ug IRF5 Polyclonal antibody (10547-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 10547-1-PBS in a different storage buffer formulation.

IRF5 Polyclonal antibody, PBS Only

IRF5 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Cat No. 10547-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Synonyms

interferon regulatory factor 5, IRF 5, IRF-5 , SLEB10

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10547-1-PBS targets IRF5 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag0841

Product name: Recombinant human IRF5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 173-498 aa of BC004201

Sequence: TLRPPTLQPPTLQPPVVLGPPAPDPSPLAPPPGNPAGFRELLSEVLEPGPLPASLPPAGEQLLPDLLISPHMLPLTDLEIKFQYRGRPPRALTISNPHGCRLFYSQLEATQEQVELFGPISLEQVRFPSPEDIPSDKQRFYTNQLLDVLDRGLILQLQGQDLYAIRLCQCKVFWSGPCASAHDSCPNPIQREVKTKLFSLEHFLNELILFQKGQTNTPPPFEIFFCFGEEWPDRKPREKKLITVQVVPVAARLLLEMFSGELSWSADSIRLQISNPDLKDRMVEQFKELHHIWQSQQRLQPVAQAPPGAGLGVGQGPWPMHPAGMQ

相同性解析による交差性が予測される生物種
Full Name interferon regulatory factor 5
Calculated molecular weight 56 kDa
Observed molecular weight 56 kDa
GenBank accession numberBC004201
Gene Symbol IRF5
Gene ID (NCBI) 3663
RRIDAB_2280474
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13568
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

IRF5, also named as SLEB10, contians one IRF tryptophan pentad repeat DNA-binding domain and belongs to the IRF family. It is a transcription factor involved in the induction of interferons IFNA and INFB and inflammatory cytokines upon virus infection. It is activated by TLR7 or TLR8 signaling. Genetic variations in IRF5 are associated with susceptibility to inflammatory bowel disease type 14 (IBD14) and systemic lupus erythematosus type 10 (SLEB10). Alternative splice variant encoding different isoforms exist. Catalog#10547-1-AP is a rabbit polyclonal antibody raised against the C-terminal of human IRF5.

||
New chat

Able™

正在加载，请稍候...