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Validation Data Gallery

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  • Western Blot (WB) analysis of human spleen tissue using IRF4 Monoclonal antibody (66451-1-Ig)

    WB analysis of human spleen using 66451-1-Ig (same clone as 66451-1-PBS)

    human spleen tissue were subjected to SDS PAGE followed by western blot with 66451-1-Ig (IRF4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66451-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using IRF4 Monoclonal antibody (66451-1-Ig)

    WB analysis using 66451-1-Ig (same clone as 66451-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66451-1-Ig (IRF4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66451-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using IRF4 Monoclonal antibody (66451-1-Ig)

    WB analysis using 66451-1-Ig (same clone as 66451-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66451-1-Ig (IRF4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66451-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig spleen tissue using IRF4 Monoclonal antibody (66451-1-Ig)

    WB analysis of pig spleen using 66451-1-Ig (same clone as 66451-1-PBS)

    pig spleen tissue were subjected to SDS PAGE followed by western blot with 66451-1-Ig (IRF4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66451-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using IRF4 Monoclonal antibody (66451-1-Ig)

    IHC staining of human tonsillitis using 66451-1-Ig (same clone as 66451-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66451-1-Ig (IRF4 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66451-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using IRF4 Monoclonal antibody (66451-1-Ig)

    IHC staining of human tonsillitis using 66451-1-Ig (same clone as 66451-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66451-1-Ig (IRF4 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66451-1-PBS in a different storage buffer formulation.

IRF4 Monoclonal antibody, PBS Only

IRF4 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66451-1-PBS
Clone No.1A12D5

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, pig

Applications

WB, IHC, Indirect ELISA

Synonyms

MUM1/IRF4, 1A12D5, interferon regulatory factor 4, IRF 4, IRF-4

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66451-1-PBS targets IRF4 in WB, IHC, Indirect ELISA applications and shows reactivity with human, rat, pig samples.

Tested Reactivity human, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag25203

Product name: Recombinant human IRF4 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-451 aa of BC015752

Sequence: MNLEGGGRGGEFGMSAVSCGNGKLRQWLIDQIDSGKYPGLVWENEEKSIFRIPWKHAGKQDYNREEDAALFKAWALFKGKFREGIDKPDPPTWKTRLRCALNKSNDFEELVERSQLDISDPYKVYRIVPEGAKKGAKQLTLEDPQMSMSHPYTMTTPYPSLPAQQVHNYMMPPLDRSWRDYVPDQPHPEIPYQCPMTFGPRGHHWQGPACENGCQVTGTFYACAPPESQAPGVPTEPSIRSAEALAFSDCRLHICLYYREILVKELTTSSPEGCRISHGHTYDASNLDQVLFPYPEDNGQRKNIEKLLSHLERGVVLWMAPDGLYAKRLCQSRIYWDGPLALCNDRPNKLERDQTCKLFDTQQFLSELQAFAHHGRSLPRFQVTLCFGEEFPDPQRQRKLITAHVEPLLARQLYYFAQQNSGHFLRGYDLPEHISNPEDYHRSIRHSSIQE

相同性解析による交差性が予測される生物種
Full Name IRF 4
Calculated molecular weight 52 kDa
Observed molecular weight 48-52 kDa
GenBank accession numberBC015752
Gene Symbol IRF4
Gene ID (NCBI) 3662
ENSEMBL Gene IDENSG00000137265
RRIDAB_2881820
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDQ15306
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

IF regulatory factor 4 (IRF4) is a member of the IRF family of transcription factors, expressed in most cell types of the immune system. IRF4 is a transcription factor essential for the development of T helper-2 (Th2) cells, IL17-producing Th17 cells, and IL9-producing Th9 cells, as well as dendritic cell (DC). It binds to and activates the IF-stimulated response element (ISRE) of the MHC class I promoter, and may have a role in ISRE-targeted signal transduction mechanisms specific to lymphoid cells.

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