Validation Data Gallery
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Tested Applications
| Positive WB detected in | HT-29 cells, LoVo cells, A549 cells, HeLa cells, HEK-293 cells, HepG2 cells, K-562 cells |
| Positive IF/ICC detected in | U2OS cells, A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
61030-1-Ig targets IRF2BP2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | interferon regulatory factor 2 binding protein 2 |
| Calculated molecular weight | 61 kDa |
| Observed molecular weight | 65-70 kDa |
| GenBank accession number | NM_182972 |
| Gene Symbol | IRF2BP2 |
| Gene ID (NCBI) | 359948 |
| RRID | AB_3743262 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q7Z5L9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
IRF2BP2 belongs to the IRF2BP family. IRF2BP2 acts as a transcriptional repressor and as a transcriptional corepressor in a IRF2-dependent manner. This repression is not mediated at least in part by histone deacetylase activities.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for IRF2BP2 antibody 61030-1-Ig | Download protocol |
| WB protocol for IRF2BP2 antibody 61030-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |