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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using IRF2BP1 Polyclonal antibody (13698-1-AP)

    WB analysis of HeLa using 13698-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 13698-1-AP (IRF2BP1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat lymph tissue using IRF2BP1 Polyclonal antibody (13698-1-AP)

    WB analysis of rat lymph using 13698-1-AP

    rat lymph tissue were subjected to SDS PAGE followed by western blot with 13698-1-AP (IRF2BP1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using IRF2BP1 Polyclonal antibody (13698-1-AP)

    WB analysis of HEK-293 using 13698-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 13698-1-AP (IRF2BP1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using IRF2BP1 Polyclonal antibody (13698-1-AP)

    WB analysis of mouse brain using 13698-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 13698-1-AP (IRF2BP1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using IRF2BP1 Polyclonal antibody (13698-1-AP)

    WB analysis of Jurkat using 13698-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 13698-1-AP (IRF2BP1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using IRF2BP1 Polyclonal antibody (13698-1-AP)

    IP experiment of HEK-293 using 13698-1-AP

    IP result of anti-IRF2BP1 (IP:13698-1-AP, 4ug; Detection:13698-1-AP 1:500) with HEK-293 cells lysate 2000ug.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using IRF2BP1 Polyclonal antibody (13698-1-AP)

    IHC staining of human breast cancer using 13698-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13698-1-AP (IRF2BP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using IRF2BP1 Polyclonal antibody (13698-1-AP)

    IHC staining of human breast cancer using 13698-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13698-1-AP (IRF2BP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using IRF2BP1 Polyclonal antibody (13698-1-AP)

    IF Staining of HeLa using 13698-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 13698-1-AP (IRF2BP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using IRF2BP1 Polyclonal antibody (13698-1-AP)

    IF Staining of HeLa using 13698-1-AP

    Immunofluorescent analysis of Hela cells, using IRF2BP1 antibody 13698-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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IRF2BP1 Polyclonal antibody

IRF2BP1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 13698-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ChIP, ELISA

Synonyms

EC:2.3.2.27, Interferon regulatory factor 2-binding protein 1, IRF 2 binding protein 1, IRF 2BP1, IRF-2-binding protein 1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, Jurkat cells, mouse brain tissue, rat lymph tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13698-1-AP targets IRF2BP1 in WB, IHC, IF/ICC, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IRF2BP1 fusion protein Ag4596 相同性解析による交差性が予測される生物種
Full Name interferon regulatory factor 2 binding protein 1
Calculated molecular weight 584 aa, 62 kDa
Observed molecular weight 62-72 kDa
GenBank accession numberBC038222
Gene Symbol IRF2BP1
Gene ID (NCBI) 26145
RRIDAB_2126853
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IU81
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IRF2BP1 inhibits IRF2-mediated transcriptional activation by interacting with IRF2. In epidermal growth factor receptor (EGFR) signaling, transient deSUMOylation of IRF2BP1 is essential for the proper expression of immediate early genes such as DUSP1 and ATF3. IRF2BP1 is also involved in the TGF-β signaling pathway by controlling the accumulation of cPML in the cytoplasm, which in turn affects the phosphorylation of Smad2 and the assembly of the Smad2-receptor complex.

Protocols

Product Specific Protocols
WB protocol for IRF2BP1 antibody 13698-1-APDownload protocol
IHC protocol for IRF2BP1 antibody 13698-1-APDownload protocol
IF protocol for IRF2BP1 antibody 13698-1-APDownload protocol
IP protocol for IRF2BP1 antibody 13698-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF,ChIP

EMBO Rep

Transient deSUMOylation of IRF2BP proteins controls early transcription in EGFR signaling.

Authors - Sina V Barysch
View Article
humanWB

Cell Death Dis

The Sumo proteome of proliferating and neuronal-differentiating cells reveals Utf1 among key Sumo targets involved in neurogenesis.

Authors - Juan F Correa-Vázquez
View Article