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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IRAK4 Monoclonal antibody (67180-1-Ig)

    WB analysis using 67180-1-Ig (same clone as 67180-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67180-1-Ig (IRAK4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67180-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using IRAK4 Monoclonal antibody (67180-1-Ig)

    IHC staining of mouse pancreas using 67180-1-Ig (same clone as 67180-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 67180-1-Ig (IRAK4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67180-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using IRAK4 Monoclonal antibody (67180-1-Ig)

    IHC staining of human placenta using 67180-1-Ig (same clone as 67180-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67180-1-Ig (IRAK4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67180-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using IRAK4 Monoclonal antibody (67180-1-Ig)

    IHC staining of human placenta using 67180-1-Ig (same clone as 67180-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67180-1-Ig (IRAK4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67180-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using IRAK4 Monoclonal antibody (67180-1-Ig)

    IHC staining of mouse kidney using 67180-1-Ig (same clone as 67180-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 67180-1-Ig (IRAK4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67180-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat pancreas tissue using IRAK4 Monoclonal antibody (67180-1-Ig)

    IHC staining of rat pancreas using 67180-1-Ig (same clone as 67180-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 67180-1-Ig (IRAK4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67180-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat stomach tissue using IRAK4 Monoclonal antibody (67180-1-Ig)

    IHC staining of rat stomach using 67180-1-Ig (same clone as 67180-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 67180-1-Ig (IRAK4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67180-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using IRAK4 Monoclonal antibody (67180-1-Ig)

    IF Staining of HepG2 using 67180-1-Ig (same clone as 67180-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using IRAK4 antibody (67180-1-Ig, Clone: 1A4C4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 67180-1-PBS in a different storage buffer formulation.

IRAK4 Monoclonal antibody, PBS Only

IRAK4 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67180-1-PBS
Clone No.1A4C4

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

1A4C4, EC:2.7.11.1, Interleukin-1 receptor-associated kinase 4, IPD1, IRAK 4

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67180-1-PBS targets IRAK4 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag29174

Product name: Recombinant human IRAK4 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-200 aa of BC013316

Sequence: MNKPITPSTYVRCLNVGLIRKLSDFIDPQEGWKKLAVAIKKPSGDDRYNQFHIRRFEALLQTGKSPTSELLFDWGTTNCTVGDLVDLLIQNEFFAPASLLLPDAVPKTANTLPSKEAITVQQKQMPFCDKDRTLMTPVQNLEQSYMPPDSSSPENKSLEVSDTRFHSFSFYELKNVTNNFDERPISVGGNKMGEGGFGVV

相同性解析による交差性が予測される生物種
Full Name interleukin-1 receptor-associated kinase 4
Calculated molecular weight 52 kDa
Observed molecular weight 52 kDa
GenBank accession numberBC013316
Gene Symbol IRAK4
Gene ID (NCBI) 51135
RRIDAB_2882476
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ9NWZ3
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.