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Validation Data Gallery

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  • Western Blot (WB) analysis of THP-1 cells using IRAK3 Recombinant antibody (83876-2-RR)

    WB analysis of THP-1 using 83876-2-RR

    THP-1 cells were subjected to SDS PAGE followed by western blot with 83876-2-RR (IRAK3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of THP-1 cells using IRAK3 Recombinant monoclonal antibody (83876-2-RR)

    WB analysis of THP-1 using 83876-2-RR

    WB result of IRAK3 antibody (83876-2-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-IRAK3 transfected THP-1 cells.

  • Western Blot (WB) analysis of various lysates using IRAK3 Recombinant antibody (83876-2-RR)

    WB analysis using 83876-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83876-2-RR (IRAK3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse lung tissue using IRAK3 Recombinant antibody (83876-2-RR)

    WB analysis of mouse lung using 83876-2-RR

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 83876-2-RR (IRAK3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of THP-1 cells using IRAK3 Recombinant antibody (83876-2-RR)

    IF Staining of THP-1 using 83876-2-RR

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using IRAK3 antibody (83876-2-RR, Clone: 240975C5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 83876-2-RR

    Affinity and Kinetic Characterization of 83876-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83876-2-RR against Human IRAK3 were performed. The affinity constant is below 1 pM.

  • Featured Product
  • KD/KO Validated

IRAK3 Recombinant monoclonal antibody

IRAK3 Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 83876-2-RR
Clone No.240975C5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

240975C5, ASRT5, IL-1 receptor-associated kinase M, Inactive IL-1 receptor-associated kinase 3, Interleukin-1 receptor-associated kinase 3

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inTHP-1 cells, HL-60 cells, mouse lung tissue
Positive IF/ICC detected inTHP-1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83876-2-RR targets IRAK3 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag4959

Product name: Recombinant human IRAK3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 251-596 aa of BC057800

Sequence: DRLQCVGDTAPLPWHIRIGILIGISKAIHYLHNVQPCSVICGSISSANILLDDQFQPKLTDFAMAHFRSHLEHQSCTINMTSSSSKHLWYMPEEYIRQGKLSIKTDVYSFGIVIMEVLTGCRVVLDDPKHIQLRDLLRELMEKRGLDSCLSFLDKKVPPCPRNFSAKLFCLAGRCAATRAKLRPSMDEVLNTLESTQASLYFAEDPPTSLKSFRCPSPLFLENVPSIPVEDDESQNNNLLPSDEGLRIDRMTQKTPFECSQSEVMFLSLDKKPESKRNEEACNMPSSSCEESWFPKYIVPSQDLRPYKVNIDPSSEAPGHSCRSRPVESSCSSKFSWDEYEQYKKE

相同性解析による交差性が予測される生物種
Full Name interleukin-1 receptor-associated kinase 3
Calculated molecular weight 68 kDa
Observed molecular weight68 kDa
GenBank accession numberBC057800
Gene Symbol IRAK3
Gene ID (NCBI) 11213
RRIDAB_3671461
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ9Y616
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IRAK3 (Interleukin-1 receptor-associated kinase-3, also named IRAKM) as a kinase-deficient member of the TLR/IRAK family has been shown to negatively regulate Toll-like receptor (TLR)-mediated cell signaling (PMID: 33590831).

Protocols

Product Specific Protocols
IF protocol for IRAK3 antibody 83876-2-RRDownload protocol
WB protocol for IRAK3 antibody 83876-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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