Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse kidney tissue, mouse spleen tissue, rat kidney tissue, rat spleen tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 1 publications below |
Product Information
28200-1-AP targets IRAK2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag28179 Product name: Recombinant human IRAK2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 14-251 aa of BC125184 Sequence: DDLCRNMDALSEWDWMEFASYVITDLTQLRKIKSMERVQGVSITRELLWWWGMRQATVQQLVDLLCRLELYRAAQIILNWKPAPEIRCPIPAFPDSVKPEKPLAASVRKAEDEQEEGQPVRMATFPGPGSSPARAHQPAFLQPPEEDAPHSLRSDLPTSSDSKDFSTSIPKQEKLLSLAGDSLFWSEADVVQATDDFNQNRKISQGTFADVYRGHRHGKPFVFKKLRETACSSPGSIE 相同性解析による交差性が予測される生物種 |
| Full Name | interleukin-1 receptor-associated kinase 2 |
| Calculated molecular weight | 625 aa, 69 kDa |
| Observed molecular weight | 69 kDa |
| GenBank accession number | BC125184 |
| Gene Symbol | IRAK2 |
| Gene ID (NCBI) | 3656 |
| RRID | AB_3742284 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O43187 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
IRAK2 (Interleukin-1 receptor-associated kinase 2) is a crucial protein involved in innate immune signaling and has diverse roles in various biological processes. IRAK2 belongs to the IRAK family of proteins, which share an N-terminal death domain (DD) that enables interactions with the DD-containing MyD88, and a central kinase domain (KD) (PMID: 24973222). IRAK2 has been implicated in various cancers. It is highly expressed in pancreatic cancer patients and is associated with poor prognosis. IRAK2 knockdown impairs pancreatic cancer cell proliferation (PMID: 37108068).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for IRAK2 antibody 28200-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |