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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IRAK2 Polyclonal antibody (28200-1-AP)

    WB analysis using 28200-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28200-1-AP (IRAK2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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IRAK2 Polyclonal antibody

IRAK2 Polyclonal Antibody for WB, ELISA
Cat No. 28200-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

Interleukin-1 receptor-associated kinase-like 2, IRAK 2, IRAK-2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse kidney tissue, mouse spleen tissue, rat kidney tissue, rat spleen tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28200-1-AP targets IRAK2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IRAK2 fusion protein Ag28179 相同性解析による交差性が予測される生物種
Full Name interleukin-1 receptor-associated kinase 2
Calculated molecular weight 625 aa, 69 kDa
Observed molecular weight69 kDa
GenBank accession numberBC125184
Gene Symbol IRAK2
Gene ID (NCBI) 3656
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO43187
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IRAK2 (Interleukin-1 receptor-associated kinase 2) is a crucial protein involved in innate immune signaling and has diverse roles in various biological processes. IRAK2 belongs to the IRAK family of proteins, which share an N-terminal death domain (DD) that enables interactions with the DD-containing MyD88, and a central kinase domain (KD) (PMID: 24973222). IRAK2 has been implicated in various cancers. It is highly expressed in pancreatic cancer patients and is associated with poor prognosis. IRAK2 knockdown impairs pancreatic cancer cell proliferation (PMID: 37108068).

Protocols

Product Specific Protocols
WB protocol for IRAK2 antibody 28200-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols