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Validation Data Gallery

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  • Western Blot (WB) analysis of human testis tissue using IPO11 Polyclonal antibody (14403-1-AP)

    WB analysis of human testis using 14403-1-AP (same clone as 14403-1-PBS)

    human testis tissue were subjected to SDS PAGE followed by western blot with 14403-1-AP (IPO11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14403-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse testis tissue using IPO11 Polyclonal antibody (14403-1-AP)

    IP experiment of mouse testis using 14403-1-AP (same clone as 14403-1-PBS)

    IP result of anti-IPO11 (IP:14403-1-AP, 4ug; Detection:14403-1-AP 1:1000) with mouse testis tissue lysate 2400 ug. This data was developed using the same antibody clone with 14403-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using IPO11 Polyclonal antibody (14403-1-AP)

    IHC staining of human colon cancer using 14403-1-AP (same clone as 14403-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14403-1-AP (IPO11 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14403-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using IPO11 Polyclonal antibody (14403-1-AP)

    IHC staining of human colon cancer using 14403-1-AP (same clone as 14403-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14403-1-AP (IPO11 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14403-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using IPO11 Polyclonal antibody (14403-1-AP)

    IF Staining of HeLa using 14403-1-AP (same clone as 14403-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using IPO11 antibody (14403-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 14403-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

IPO11 Polyclonal antibody, PBS Only

IPO11 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 14403-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

RanBP11, Ran-binding protein 11, Ran binding protein 11, Importin-11, importin 11

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14403-1-PBS targets IPO11 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag5659

Product name: Recombinant human IPO11 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-350 aa of BC033776

Sequence: MDLNSASTVVLQVLTQATSQDTAVLKPAEEQLKQWETQPGFYSVLLNIFTNHTLDINVRWLAVLYFKHGIDRYWRRVAPHALSEEEKTTLRAGLITNFNEPINQIATQIAVLIAKVARLDCPRQWPELIPTLIESVKVQDDLRQHRALLTFYHVTKTLASKRLAADRKLFYDLASGIYNFACSLWNHHTDTFLQEVSSGNEAAILSSLERTLLSLKVLRKLTVNGFVEPHKNMEVMGFLHGIFERLKQFLECSRSIGTDNVCRDRLEKTIILFTKVLLDFLDQHPFSFTPLIQRSLEFSVSYVFTEVGEGVTFERFIVQCMNLIKMIVKNYAYKPSKNFEDSSPETLEAH

相同性解析による交差性が予測される生物種
Full Name importin 11
Calculated molecular weight 113 kDa
Observed molecular weight 112 kDa
GenBank accession numberBC033776
Gene Symbol IPO11
Gene ID (NCBI) 51194
RRIDAB_2296214
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UI26
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Importins are members of a family of transport receptors (karyopherins) that mediate the nucleocytoplasmic transport of protein and RNA cargoes. Importin-11, is a nuclear import receptor for the ubiquitin-conjugating enzyme, UbcM2.

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