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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IP6K1 Polyclonal antibody (12057-2-AP)

    WB analysis using 12057-2-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12057-2-AP (IP6K1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human kidney tissue using IP6K1 Polyclonal antibody (12057-2-AP)

    WB analysis of human kidney using 12057-2-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 12057-2-AP (IP6K1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human liver tissue using IP6K1 Polyclonal antibody (12057-2-AP)

    WB analysis of human liver using 12057-2-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 12057-2-AP (IP6K1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse kidney tissue using IP6K1 Polyclonal antibody (12057-2-AP)

    IP experiment of mouse kidney using 12057-2-AP

    IP result of anti-IP6K1 (IP:12057-2-AP, 4ug; Detection:12057-2-AP 1:300) with mouse kidney tissue lysate 3000 ug.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using IP6K1 Polyclonal antibody (12057-2-AP)

    IHC staining of human lung cancer using 12057-2-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12057-2-AP (IP6K1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using IP6K1 Polyclonal antibody (12057-2-AP)

    IHC staining of human colon cancer using 12057-2-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12057-2-AP (IP6K1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using IP6K1 Polyclonal antibody (12057-2-AP)

    IHC staining of human colon cancer using 12057-2-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12057-2-AP (IP6K1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using IP6K1 Polyclonal antibody (12057-2-AP)

    IHC staining of human lung cancer using 12057-2-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 12057-2-AP (IP6K1 antibody) at dilution of 1:50 (under 10x lens).

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IP6K1 Polyclonal antibody

IP6K1 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 12057-2-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, ELISA

Synonyms

IHPK1, InsP6 kinase 1, IP6K1, KIAA0263, PiUS

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA375 cells, human liver tissue, human kidney tissue, Hela cells, HepG2 cells
Positive IP detected inmouse kidney tissue
Positive IHC detected inhuman colon cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

12057-2-AP targets IP6K1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IP6K1 fusion protein Ag2687 相同性解析による交差性が予測される生物種
Full Name inositol hexakisphosphate kinase 1
Calculated molecular weight 441 aa, 50 kDa
Observed molecular weight 54 kDa
GenBank accession numberBC012944
Gene Symbol IP6K1
Gene ID (NCBI) 9807
RRIDAB_2127529
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ92551
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for IP6K1 antibody 12057-2-APDownload protocol
IHC protocol for IP6K1 antibody 12057-2-APDownload protocol
IP protocol for IP6K1 antibody 12057-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
pigWB

Pediatr Res

Preterm birth alters the feeding-induced activation of Akt signaling in the muscle of neonatal piglets

Authors - Agus Suryawan
View Article