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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using INTS4 Recombinant antibody (83590-5-RR)

    IF Staining of HeLa using 83590-5-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using INTS4 antibody (83590-5-RR, Clone: 240579H4 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using INTS4 Recombinant antibody (83590-5-RR)

    IF Staining of U2OS using 83590-5-RR

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using INTS4 antibody (83590-5-RR, Clone: 240579H4 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of A431 cells using INTS4 Recombinant antibody (83590-5-RR)

    FC experiment of A431 using 83590-5-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug INTS4 Recombinant antibody (83590-5-RR, Clone:240579H4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83590-5-RR

    Affinity and Kinetic Characterization of 83590-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83590-5-RR against Human INTS4 were performed. The affinity constant is 0.146 nM.

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INTS4 Recombinant antibody

INTS4 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 83590-5-RR
Clone No.240579H4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Synonyms

MSTP093, MST093, Int4, 240579H4

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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Tested Applications

Positive IF/ICC detected inU2OS cells, HeLa cells
Positive FC (Intra) detected inA431 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83590-5-RR targets INTS4 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen INTS4 fusion protein Ag9116 相同性解析による交差性が予測される生物種
Full Name integrator complex subunit 4
Calculated molecular weight963aa,108 kDa; 505aa,57 kDa
GenBank accession numberBC009995
Gene Symbol INTS4
Gene ID (NCBI) 92105
RRIDAB_3671204
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ96HW7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Integrator complex subunit 4 (INTS4), a helical repeat protein, plays a key role in stabilizing nuclease domains and other components. INTS4 is a specific and conserved interaction partner of INTS9/11 heterodimer that does not interact with either subunit individually (PMID: 29471365). INTS4 plays a role in achieving a compact nuclease/pseudonuclease configuration resembling that observed for CPSF73/100 in the histone-processing machinery (PMID: 33548203).

Protocols

Product Specific Protocols
IF protocol for INTS4 antibody 83590-5-RRDownload protocol
FC protocol for INTS4 antibody 83590-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols