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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using INTS4 Polyclonal antibody (16130-1-AP)

    WB analysis using 16130-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16130-1-AP (INTS4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using INTS4 Polyclonal antibody (16130-1-AP)

    WB analysis of HepG2 using 16130-1-AP

    WB result of INTS4 antibody (16130-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-INTS4 transfected HepG2 cells.

  • Featured Product
  • KD/KO Validated

INTS4 Polyclonal antibody

INTS4 Polyclonal Antibody for WB, ELISA
Cat No. 16130-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

Int4, MST093, MSTP093

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, Jurkat cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16130-1-AP targets INTS4 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag9116

Product name: Recombinant human INTS4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-331 aa of BC009995

Sequence: MAAHLKKRVYEEFTKVVQPQEEIATKKLRLTKPSKSAALHIDLCKATSPADALQYLLQFARKPVEAESVEGVVRILLEHYYKENDPSVRLKIASLLGLLSKTAGFSPDCIMDDAINILQNEKSHQVLAQLLDTLLAIGTKLPENQAIQMRLVDVACKHLTDTSHGVRNKCLQLLGNLGSLEKSVTKDAEGLAARDVQKIIGDYFSDQDPRVRTAAIKAMLQLHERGLKLHQTIYNQACKLLSDDYEQVRSAAVQLIWVVSQLYPESIVPIPSSNEEIRLVDDAFGKICHMVSDGSWVVRVQAAKLLGSMEQVSSHFLEQTLDKKLMSDLRR

相同性解析による交差性が予測される生物種
Full Name integrator complex subunit 4
Calculated molecular weight963aa,108 kDa; 505aa,57 kDa
Observed molecular weight 108 kDa
GenBank accession numberBC009995
Gene Symbol INTS4
Gene ID (NCBI) 92105
RRIDAB_2296175
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96HW7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Integrator complex subunit 4 (INTS4), a helical repeat protein, plays a key role in stabilizing nuclease domains and other components. INTS4 is a specific and conserved interaction partner of INTS9/11 heterodimer that does not interact with either subunit individually (PMID: 29471365). INTS4 plays a role in achieving a compact nuclease/pseudonuclease configuration resembling that observed for CPSF73/100 in the histone-processing machinery (PMID: 33548203).

Protocols

Product Specific Protocols
WB protocol for INTS4 antibody 16130-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols