Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells, HepG2 cells, mouse testis tissue, rat testis tissue |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 10 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
| ChIP | See 2 publications below |
Product Information
16620-1-AP targets INTS3 in WB, IHC, IF, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9917 Product name: Recombinant human INTS3 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 91-445 aa of BC025254 Sequence: YHLLYYLRASKAAAGKMNLYESFAQATQLGDLHTCLMMDMKACQEDDVRLLCHLTPSIYTEFPDETLRSGELLNMIVAVIDSAQLQELVCHVMMGNLVMFRKDSVLNILIQSLDWETFEQYCAWQLFLAHNIPLETIIPILQHLKYKEHPEALSCLLLQLRREKPSEEMVKMVLSRPCHPDDQFTTSILRHWCMKHDELLAEHIKSLLIKNNSLPRKRQSLRSSSSKLAQLTLEQILEHLDNLRLNLTNTKQNFFSQTPILQALQHVQASCDEAHKMKFSDLFSLAEEYEDSSTKPPKSRRKAALSSPRSRKNATQPPNAEEESGSSSASEEEDTKPKPTKRKRKGSSAVGSDSD 相同性解析による交差性が予測される生物種 |
| Full Name | integrator complex subunit 3 |
| Calculated molecular weight | 118 kDa |
| Observed molecular weight | 118 kDa |
| GenBank accession number | BC025254 |
| Gene Symbol | INTS3 |
| Gene ID (NCBI) | 65123 |
| RRID | AB_2127274 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q68E01 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
INTS3 is a member of the integrator complex characterised as an RNA polymerase II (RNAPII) C-terminal domain binding factor involved in the processing of small nuclear RNAs (snRNAs) (PMID: 16239144). INTS3 was overexpressed in colorectal cancer (CRC) patients and tumour cell lines and inhibited CRC apoptosis by promoting the degradation of pro-apoptotic gene transcripts (PMID: 38665208).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for INTS3 antibody 16620-1-AP | Download protocol |
| IP protocol for INTS3 antibody 16620-1-AP | Download protocol |
| WB protocol for INTS3 antibody 16620-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Science Identification of Integrator-PP2A complex (INTAC), an RNA polymerase II phosphatase. | ||
Cell The PP2A-Integrator-CDK9 axis fine-tunes transcription and can be targeted therapeutically in cancer. | ||
Mol Cell PGC-1α senses the CBC of pre-mRNA to dictate the fate of promoter-proximally paused RNAPII | ||
Mol Cell Integrator Recruits Protein Phosphatase 2A to Prevent Pause Release and Facilitate Transcription Termination. | ||
Mol Cell Deregulated Expression of Mammalian lncRNA through Loss of SPT6 Induces R-Loop Formation, Replication Stress, and Cellular Senescence. | ||
Nat Commun INTS13 variants causing a recessive developmental ciliopathy disrupt assembly of the Integrator complex |
