Validation Data Gallery
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Tested Applications
| Positive WB detected in | HepG2 cells |
| Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 4 publications below |
Product Information
16455-1-AP targets INTS12 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | INTS12 fusion protein Ag9403 相同性解析による交差性が予測される生物種 |
| Full Name | integrator complex subunit 12 |
| Calculated molecular weight | 462 aa, 49 kDa |
| Observed molecular weight | 49 kDa |
| GenBank accession number | BC014442 |
| Gene Symbol | INTS12 |
| Gene ID (NCBI) | 57117 |
| RRID | AB_2127263 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96CB8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for INTS12 antibody 16455-1-AP | Download protocol |
| IHC protocol for INTS12 antibody 16455-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cell Redundant pathways for removal of defective RNA polymerase II complexes at a promoter-proximal pause checkpoint | ||
Nat Commun INTS13 variants causing a recessive developmental ciliopathy disrupt assembly of the Integrator complex | ||
PLoS Genet Human mutations in integrator complex subunits link transcriptome integrity to brain development. | ||
Cell Rep A homozygous variant in INTS11 links mitosis and neurogenesis defects to a severe neurodevelopmental disorder |