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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse kidney tissue using INSRR Polyclonal antibody (55112-1-AP)

    WB analysis of mouse kidney using 55112-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 55112-1-AP (INSRR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using INSRR Polyclonal antibody (55112-1-AP)

    WB analysis of human brain using 55112-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 55112-1-AP (INSRR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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INSRR Polyclonal antibody

INSRR Polyclonal Antibody for WB, ELISA
Cat No. 55112-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

INSRR, IR related receptor, IRR

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inmouse kidney tissue, human brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55112-1-AP targets INSRR in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name IR related receptor
Calculated molecular weight 144 kDa
Observed molecular weight 150-155 kDa
GenBank accession numberNM_014215
Gene Symbol INSRR
Gene ID (NCBI) 3645
RRIDAB_10859077
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP14616
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

INSRR, also named as IRR, belongs to the protein kinase superfamily, Tyr protein kinase family and INS receptor subfamily. It probably binds an INS related protein and has a tyrosine-protein kinase activity. It phosphorylates the INS receptor substrates IRS-1 and IRS-2. INSRR is a heterotetrameric transmembrane receptor composed of two alpha and two beta chains linked by disulfide bonds. INSRR shares high homology with the INS (IR) and the IGF-1R, but doesn't bind any of IR and IGF-1R known ligands. The antibody is specific to INSRR.

Protocols

Product Specific Protocols
WB protocol for INSRR antibody 55112-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols