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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using INSR Recombinant antibody (84467-2-RR)

    WB analysis using 84467-2-RR

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 84467-2-RR (INSR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using INSR/CD220 Recombinant antibody (84467-2-RR)

    IHC staining of human breast cancer using 84467-2-RR

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 84467-2-RR (INSR/CD220 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using INSR/CD220 Recombinant antibody (84467-2-RR)

    IF Staining of MCF-7 using 84467-2-RR

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using INSR/CD220 antibody (84467-2-RR, Clone: 241687H7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84467-2-RR

    Affinity and Kinetic Characterization of 84467-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84467-2-RR against Human INSR were performed. The affinity constant is 0.189 nM.

INSR/CD220 Recombinant monoclonal antibody

INSR/CD220 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 84467-2-RR
Clone No.241687H7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

INSR, 241687H7, CD220, EC:2.7.10.1, HHF5

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inMCF-7 cells, HEK-293T cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84467-2-RR targets INSR/CD220 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

Recombinant Protein

相同性解析による交差性が予測される生物種
Full Name INSR
Observed molecular weight95 kDa
GenBank accession numberBC117172
Gene Symbol INSR
Gene ID (NCBI) 3643
RRIDAB_3671990
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP06213
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The biological effects of INS are mediated by a membrane-spanning cell surface receptor that has been shown to consist of disulfide-linked alpha-subunits (135 kDa) and beta-subunits (95 kDa) that are cleaved products of a common precursor. The binding of INS to the receptor extracellular domain results in intracellular activation of a tyrosine-specific kinase activity and the generation of signals that determine the cellular response (PMID: 2369896).

Protocols

Product Specific Protocols
IF protocol for INSR/CD220 antibody 84467-2-RRDownload protocol
IHC protocol for INSR/CD220 antibody 84467-2-RRDownload protocol
WB protocol for INSR/CD220 antibody 84467-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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