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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse liver tissue using INSC Polyclonal antibody (20973-1-AP)

    WB analysis of mouse liver using 20973-1-AP (same clone as 20973-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 20973-1-AP (INSC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20973-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse liver tissue using INSC Polyclonal antibody (20973-1-AP)

    WB analysis of mouse liver using 20973-1-AP (same clone as 20973-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 20973-1-AP (INSC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20973-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse kidney tissue using INSC Polyclonal antibody (20973-1-AP)

    WB analysis of mouse kidney using 20973-1-AP (same clone as 20973-1-PBS)

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20973-1-AP (INSC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20973-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse kidney tissue using INSC Polyclonal antibody (20973-1-AP)

    WB analysis of mouse kidney using 20973-1-AP (same clone as 20973-1-PBS)

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20973-1-AP (INSC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20973-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using INSC Polyclonal antibody (20973-1-AP)

    IHC staining of human kidney using 20973-1-AP (same clone as 20973-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 20973-1-AP (INSC antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20973-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using INSC Polyclonal antibody (20973-1-AP)

    IHC staining of human kidney using 20973-1-AP (same clone as 20973-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 20973-1-AP (INSC antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20973-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using INSC Polyclonal antibody (20973-1-AP)

    IHC staining of human liver using 20973-1-AP (same clone as 20973-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver using 20973-1-AP (INSC antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 20973-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using INSC Polyclonal antibody (20973-1-AP)

    IHC staining of human liver using 20973-1-AP (same clone as 20973-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver using 20973-1-AP (INSC antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 20973-1-PBS in a different storage buffer formulation.

INSC Polyclonal antibody, PBS Only

INSC Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 20973-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Protein inscuteable homolog

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

20973-1-PBS targets INSC in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name inscuteable homolog (Drosophila)
Calculated molecular weight 63 kDa
Observed molecular weight 54 kDa
GenBank accession numberNM_001031853
Gene Symbol INSC
Gene ID (NCBI) 387755
RRIDAB_10951111
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ1MX18
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

INSC may function as an adapter linking the Par3 complex to the GPSM1/GPSM2 complex. It is involved in spindle orientation during mitosis it may regulate cell proliferation and differentiation in the developing nervous system. INSC may play a role in the asymmetric division of fibroblasts and participate in the process of stratification of the squamous epithelium. The antibody can recognize isoform1, 2,4,5 of INSC.

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