Validation Data Gallery
Filter:
Tested Applications
| Positive WB detected in | K-562 cells, NIH/3T3 cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
87161-1-RR targets INPPL1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | inositol polyphosphate phosphatase-like 1 |
| Calculated molecular weight | 1258 aa, 139 kDa |
| Observed molecular weight | 150 kDa |
| GenBank accession number | BC140853 |
| Gene Symbol | INPPL1 |
| Gene ID (NCBI) | 3636 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | O15357 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Inositol polyphosphate phosphatase-like protein 1(INPPL1) belongs to the inositol and phosphatidylinositol 5-phosphatase family, also known as SH2 domain-containing inositol 5'-phosphatase 2(SHIP2), is ubiquitous in all organizations. SHIP2 is constitutively tyrosine-phosphorylated in chronic myeloid leukemia progenitor cells. In B cells, SHIP2 was most tyrosine phosphorylated after BCR and FC-γriib crosslinking and was associated with Shc, but not after BCR stimulation alone.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for INPPL1 antibody 87161-1-RR | Download protocol |
| WB protocol for INPPL1 antibody 87161-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |