Validation Data Gallery
Filter:
Tested Applications
| Positive WB detected in | HEK-293 cells, MCF-7 cells |
| Positive IP detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
27240-1-AP targets INPP5F in WB, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | INPP5F fusion protein Ag25687 相同性解析による交差性が予測される生物種 |
| Full Name | inositol polyphosphate-5-phosphatase F |
| Calculated molecular weight | 1132 aa, 128 kDa |
| Observed molecular weight | 128-140 kDa |
| GenBank accession number | BC111493 |
| Gene Symbol | INPP5F |
| Gene ID (NCBI) | 22876 |
| RRID | AB_3095738 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y2H2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
INPP5F can negatively regulates STAT3 signaling pathway through inhibition of STAT3 phosphorylation and translocation to the nucleus.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for INPP5F antibody 27240-1-AP | Download protocol |
| IP protocol for INPP5F antibody 27240-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |