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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

INPP5E Monoclonal antibody

INPP5E Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2B3D6

Cat no : 68602-1-Ig

Synonyms

INPP5E, PPI5PIV

Validation Data Gallery

Applications:
  • WB analysis using 68602-1-Ig

    WB analysis using 68602-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68602-1-Ig (INPP5E antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis using 68602-1-Ig

    WB analysis using 68602-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68602-1-Ig (INPP5E antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of HaCaT using 68602-1-Ig

    WB analysis of HaCaT using 68602-1-Ig

    HaCaT cells were subjected to SDS PAGE followed by western blot with 68602-1-Ig (INPP5E antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inSH-SY5Y cells, HaCaT cells, HEK-293 cells, U-251 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Sample-dependent, check data in validation data gallery

Product Information

68602-1-Ig targets INPP5E in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen INPP5E fusion protein Ag11959 相同性解析による交差性が予測される生物種
Full Name inositol polyphosphate-5-phosphatase, 72 kDa
Calculated molecular weight 644 aa, 70 kDa
Observed molecular weight72 kDa
GenBank accession numberBC028032
Gene symbol INPP5E
Gene ID (NCBI) 56623
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

INPP5E(72 kDa inositol polyphosphate 5-phosphatase) converts phosphatidylinositol 3,4,5-trisphosphate (PtdIns 3,4,5-P3) to PtdIns-P2.In mouse, highest protein expression was in brain, heart, and testis, with lower expression in thymus and lung, and very little expression in kidney, spleen, and liver(PMID:10764818). Defects in INPP5E are the cause of Joubert syndrome type 1 (JBTS1) and mental retardation-truncal obesity-retinal dystrophy-micropenis (MORMS)(PMID:19668215).

Protocols

Product Specific Protocols
WB protocol for INPP5E antibody 68602-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols