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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using INPP5E Polyclonal antibody (29353-1-AP)

    WB analysis using 29353-1-AP (same clone as 29353-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29353-1-AP (INPP5E antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29353-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of ARPE-19 cells using INPP5E Polyclonal antibody (29353-1-AP)

    IF Staining of ARPE-19 using 29353-1-AP (same clone as 29353-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed ARPE-19 cells using INPP5E antibody (29353-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 29353-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of ARPE-19 cells using INPP5E Polyclonal antibody (29353-1-AP)

    IF Staining of ARPE-19 using 29353-1-AP (same clone as 29353-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed ARPE-19 cells using INPP5E antibody (29353-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 29353-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

29353-1-PBS targets INPP5E in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen INPP5E fusion protein Ag30305 相同性解析による交差性が予測される生物種
Full Name inositol polyphosphate-5-phosphatase, 72 kDa
Calculated molecular weight 644 aa, 70 kDa
Observed molecular weight70 kDa
GenBank accession numberBC028032
Gene Symbol INPP5E
Gene ID (NCBI) 56623
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NRR6
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

INPP5E(72 kDa inositol polyphosphate 5-phosphatase) converts phosphatidylinositol 3,4,5-trisphosphate (PtdIns 3,4,5-P3) to PtdIns-P2.In mouse, highest protein expression was in brain, heart, and testis, with lower expression in thymus and lung, and very little expression in kidney, spleen, and liver(PMID:10764818). Defects in INPP5E are the cause of Joubert syndrome type 1 (JBTS1) and mental retardation-truncal obesity-retinal dystrophy-micropenis (MORMS)(PMID:19668215). It has 2 isoforms with the molecular mass of 66 kDa and 70 kDa.