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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using INPP5A Recombinant antibody (86001-1-RR)

    WB analysis using 86001-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86001-1-RR (INPP5A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 86001-1-RR

    Affinity and Kinetic Characterization of 86001-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86001-1-RR against Human INPP5A were performed. The affinity constant is below 1 pM.

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INPP5A Recombinant antibody

INPP5A Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 86001-1-RR
Clone No.250584C3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

43 kDa inositol polyphosphate 5-phosphatase, 5PTASE, EC:3.1.3.56, Inositol polyphosphate-5-phosphatase A, Type I inositol 1,4,5-trisphosphate 5-phosphatase

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, fetal human brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

86001-1-RR targets INPP5A in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen INPP5A fusion protein Ag16380 相同性解析による交差性が予測される生物種
Full Name inositol polyphosphate-5-phosphatase, 40kDa
Calculated molecular weight 412 aa, 48 kDa
GenBank accession numberBC096280
Gene Symbol INPP5A
Gene ID (NCBI) 3632
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ14642
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

INPP5A (Inositol Polyphosphate-5-Phosphatase A) is a membrane-bound type I inositol 1,4,5-trisphosphate 5-phosphatase that is involved in the mobilization of intracellular calcium ions and the regulation of cellular responses to stimuli. It terminates calcium signaling by hydrolyzing inositol 1,4,5-trisphosphate (InsP3) and inositol 1,3,4,5-tetrakisphosphate (InsP4). INPP5A plays important roles in various cellular processes, including cell proliferation, apoptosis, vesicular transport, and insulin signaling. Additionally, INPP5A is crucial for the survival of cerebellar Purkinje cells. In certain cancers, such as cutaneous squamous cell carcinoma (cSCC) and esophageal squamous cell carcinoma (ESCC), the expression levels of INPP5A are closely related to disease progression and prognosis.

Protocols

Product Specific Protocols
WB protocol for INPP5A antibody 86001-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols