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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using INPP4A Polyclonal antibody (12665-1-AP)

    WB analysis using 12665-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12665-1-AP (INPP4A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using INPP4A Polyclonal antibody (12665-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 12665-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 12665-1-AP (INPP4A antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using INPP4A Polyclonal antibody (12665-1-AP)

    IF Staining of A549 using 12665-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using INPP4A antibody (12665-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

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INPP4A Polyclonal antibody

INPP4A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 12665-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Type I inositol 3,4-bisphosphate 4-phosphatase, INPP4, INP4A, Inositol polyphosphate-4-phosphatase type I A, Inositol polyphosphate 4-phosphatase type I

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12665-1-AP targets INPP4A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen INPP4A fusion protein Ag3346 相同性解析による交差性が予測される生物種
Full Name inositol polyphosphate-4-phosphatase, type I, 107kDa
Calculated molecular weight 972 aa, 109 kDa
Observed molecular weight100-110 kDa
GenBank accession numberBC028361
Gene Symbol INPP4A
Gene ID (NCBI) 3631
RRIDAB_3669147
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96PE3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Inositol polyphosphate 4 phosphatase (INPP4A) belongs to the group of phosphoinositide phosphatases controlling proliferation, apoptosis, and endosome function by hydrolyzing phosphatidylinositol 3,4-bisphosphate. INPP4A is highly expressed in the central nervous system, which is shown to play a key role in glutamate excitotoxicity and cell proliferation. (PMID: 36653678, PMID: 31938306)

Protocols

Product Specific Protocols
WB protocol for INPP4A antibody 12665-1-APDownload protocol
IHC protocol for INPP4A antibody 12665-1-APDownload protocol
IF protocol for INPP4A antibody 12665-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols