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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using INO80 Monoclonal antibody (68064-1-Ig)

    WB analysis using 68064-1-Ig (same clone as 68064-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68064-1-Ig (INO80 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68064-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using INO80 Monoclonal antibody (68064-1-Ig)

    WB analysis using 68064-1-Ig (same clone as 68064-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68064-1-Ig (INO80 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68064-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using INO80 Monoclonal antibody (68064-1-Ig)

    IHC staining of human colon cancer using 68064-1-Ig (same clone as 68064-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68064-1-Ig (INO80 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68064-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using INO80 Monoclonal antibody (68064-1-Ig)

    IHC staining of human colon cancer using 68064-1-Ig (same clone as 68064-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68064-1-Ig (INO80 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68064-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of PC-12 cells using INO80 Monoclonal antibody (68064-1-Ig)

    FC experiment of PC-12 using 68064-1-Ig (same clone as 68064-1-PBS)

    1X10^6 PC-12 cells were intracellularly stained with 0.8 ug Anti-Human INO80 (68064-1-Ig, Clone:1F11C5) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.8 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 68064-1-PBS in a different storage buffer formulation.

INO80 Monoclonal antibody, PBS Only

INO80 Monoclonal Antibody for WB, IHC, FC (Intra), Indirect ELISA
Cat No. 68064-1-PBS
Clone No.1F11C5

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), Indirect ELISA

Synonyms

hINO80, EC:3.6.4.-, DNA helicase-related protein INO80, DNA helicase-related INO80 complex homolog 1, Chromatin-remodeling ATPase INO80

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68064-1-PBS targets INO80 in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag20265

Product name: Recombinant human INO80 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1217-1556 aa of BC146785

Sequence: KQVTVYRLICKGTIEERILQRAKEKSEIQRMVISGGNFKPDTLKPKEVVSLLLDDEELEKKLRLRQEEKRQQEETNRVKERKRKREKYAEKKKKEDELDGKRRKEGVNLVIPFVPSADNSNLSADGDDSFISVDSAMPSPFSEISISSELHTGSIPLDESSSDMLVIVDDPASSAPQSRATNSPASITGSVSDTVNGISIQEMPAAGRGHSARSRGRPKGSGSTAKGAGKGRSRKSTAGSAAAMAGAKAGAAAASAAAYAAYGYNVSKGISASSPLQTSLVRPAGLADFGPSSASSPLSSPLSKGNNVPGNPKNLHMTSSLAPDSLVRKQGKGTNPSGGR

相同性解析による交差性が予測される生物種
Full Name INO80 homolog (S. cerevisiae)
Calculated molecular weight 1556 aa, 177 kDa
Observed molecular weight177 kDa
GenBank accession numberBC146785
Gene Symbol INO80
Gene ID (NCBI) 54617
RRIDAB_2918805
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDQ9ULG1
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

DNA helicase INO80, also namd as INO80 or hINO80, is a 1,556 amino acid protein, which contains one helicase C-terminal domain, one helicase ATP-binding domain, and one DBINO domain. INO80 co-localizes with PCMA at replication forks during S-phase in the nucleus and belongs to the SNF2 helicase family. INO80 is a component of the chromatin remolding INO80 complex and is widely expressed in many tissues, such as brain,liver and pancreas. INO80 is involved in transcription regulation.