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Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using Inhibin beta A-Specific Monoclonal antibody (60352-1-Ig)

    WB analysis of HepG2 using 60352-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 60352-1-Ig (INHBA-Specific Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using Inhibin beta A-Specific Monoclonal antibody (60352-1-Ig)

    WB analysis of human placenta using 60352-1-Ig

    human placenta tissue were subjected to SDS PAGE followed by western blot with 60352-1-Ig (Inhibin beta A-Specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig liver tissue using Inhibin beta A-Specific Monoclonal antibody (60352-1-Ig)

    WB analysis of pig liver using 60352-1-Ig

    pig liver tissue were subjected to SDS PAGE followed by western blot with 60352-1-Ig (Inhibin beta A-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig liver tissue using Inhibin beta A-Specific Monoclonal antibody (60352-1-Ig)

    WB analysis of pig liver using 60352-1-Ig

    pig liver tissue were subjected to SDS PAGE followed by western blot with 60352-1-Ig (INHBA-Specific Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human testis tissue using Inhibin beta A-Specific Monoclonal antibody (60352-1-Ig)

    WB analysis of human testis using 60352-1-Ig

    human testis tissue were subjected to SDS PAGE followed by western blot with 60352-1-Ig (INHBA-Specific Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of L02 cells using Inhibin beta A-Specific Monoclonal antibody (60352-1-Ig)

    WB analysis of L02 using 60352-1-Ig

    L02 cells were subjected to SDS PAGE followed by western blot with 60352-1-Ig (INHBA-Specific Antibody) at dilution of 1:650 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Inhibin beta A-Specific Monoclonal antibody (60352-1-Ig)

    IHC staining of human lung cancer using 60352-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60352-1-Ig (INHBA-Specific Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Inhibin beta A-Specific Monoclonal antibody (60352-1-Ig)

    IHC staining of human lung cancer using 60352-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60352-1-Ig (INHBA-Specific Antibody) at dilution of 1:150 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human oesophagus cancer tissue using Inhibin beta A-Specific Monoclonal antibody (60352-1-Ig)

    IHC staining of human oesophagus cancer using 60352-1-Ig

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 60352-1-Ig (INHBA-Specific Antibody) at dilution of 1:150 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human oesophagus cancer tissue using Inhibin beta A-Specific Monoclonal antibody (60352-1-Ig)

    IHC staining of human oesophagus cancer using 60352-1-Ig

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 60352-1-Ig (INHBA-Specific Antibody) at dilution of 1:150 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Inhibin beta A-Specific Monoclonal antibody

Inhibin beta A-Specific Monoclonal Antibody for WB, IHC, ELISA
Cat No. 60352-1-Ig
Clone No.1F8D8
Publications(1)

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

WB, IHC, IF, ELISA

Synonyms

INHBA, Inhibin Beta A, 1F8D8, Activin beta-A chain, EDF

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells, pig liver tissue, human testis tissue, L02 cells, human placenta tissue
Positive IHC detected inhuman lung cancer tissue, human oesophagus cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

60352-1-Ig targets Inhibin beta A-Specific in WB, IHC, IF, ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name inhibin, beta A
Calculated molecular weight 47 kDa
Observed molecular weight 47 kDa
GenBank accession numberNM_002192
Gene Symbol Inhibin beta A
Gene ID (NCBI) 3624
RRIDAB_2881461
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP08476
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Inhibin βA(INHBA) is a ligand in the transforming growth factor β (TGF-β) superfamily. Inhibin βA forms a disulfide-linked homo-dimer known as activin A, which has been implicated in multiple biologic processes, including neoplastic progression. The inhibin βA can also bind the βB and α isoforms to form activin AB and inhibin, respectively.

Protocols

Product Specific Protocols
IHC protocol for Inhibin beta A-Specific antibody 60352-1-IgDownload protocol
WB protocol for Inhibin beta A-Specific antibody 60352-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

BMC Cancer

INHBA+ macrophages and Pro-inflammatory CAFs are associated with distinctive immunosuppressive tumor microenvironment in submucous Fibrosis-Derived oral squamous cell carcinoma

Authors - Simin Zhao
View Article
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