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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using ING5 Polyclonal antibody (10665-1-AP)

    WB analysis of Jurkat using 10665-1-AP (same clone as 10665-1-PBS)

    WB result of ING5 antibody (10665-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ING5 transfected Jurkat cells. This data was developed using the same antibody clone with 10665-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using ING5 Polyclonal antibody (10665-1-AP)

    WB analysis of Jurkat using 10665-1-AP (same clone as 10665-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 10665-1-AP (ING5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10665-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of PC-3 cells using ING5 Polyclonal antibody (10665-1-AP)

    WB analysis of PC-3 using 10665-1-AP (same clone as 10665-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 10665-1-AP (ING5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10665-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using ING5 Polyclonal antibody (10665-1-AP)

    WB analysis of Jurkat using 10665-1-AP (same clone as 10665-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 10665-1-AP (ING5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10665-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse kidney tissue using ING5 Polyclonal antibody (10665-1-AP)

    WB analysis of mouse kidney using 10665-1-AP (same clone as 10665-1-PBS)

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10665-1-AP (ING5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10665-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using ING5 Polyclonal antibody (10665-1-AP)

    WB analysis of HEK-293 using 10665-1-AP (same clone as 10665-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 10665-1-AP (ING5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10665-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using ING5 Polyclonal antibody (10665-1-AP)

    IP experiment of HEK-293 using 10665-1-AP (same clone as 10665-1-PBS)

    IP result of anti-ING5 (IP:10665-1-AP, 4ug; Detection:10665-1-AP 1:700) with HEK-293 cells lysate 2560ug. This data was developed using the same antibody clone with 10665-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using ING5 Polyclonal antibody (10665-1-AP)

    IHC staining of human colon cancer using 10665-1-AP (same clone as 10665-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 10665-1-AP (ING5 antibody) at dilution of 1:100 (under 25x lens). This data was developed using the same antibody clone with 10665-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using ING5 Polyclonal antibody (10665-1-AP)

    IHC staining of human thyroid cancer using 10665-1-AP (same clone as 10665-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 10665-1-AP (ING5 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10665-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using ING5 Polyclonal antibody (10665-1-AP)

    IHC staining of mouse kidney using 10665-1-AP (same clone as 10665-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 10665-1-AP (ING5 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10665-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat skin tissue using ING5 Polyclonal antibody (10665-1-AP)

    IHC staining of rat skin using 10665-1-AP (same clone as 10665-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 10665-1-AP (ING5 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10665-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using ING5 Polyclonal antibody (10665-1-AP)

    IHC staining of human ovary tumor using 10665-1-AP (same clone as 10665-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10665-1-AP (ING5 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10665-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using ING5 Polyclonal antibody (10665-1-AP)

    IHC staining of human ovary tumor using 10665-1-AP (same clone as 10665-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10665-1-AP (ING5 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 10665-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

ING5 Polyclonal antibody, PBS Only

ING5 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 10665-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Inhibitor of growth protein 5, p28ING5

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10665-1-PBS targets ING5 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag1059

Product name: Recombinant human ING5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-226 aa of BC005370

Sequence: MATAMYLEHYLDSIENLPCELQRNFQLMRELDQRTEDKKAEIDILAAEYISTVKTLSPDQRVERLQKIQNAYSKCKEYSDDKVQLAMQTYEMVDKHIRRLDADLARFEADLKDKMEGSDFESSGGRGLKKGRGQKEKRGSRGRGRRTSEEDTPKKKKHKGGSEFTDTILSVHPSDVLDMPVDPNEPTYCLCHQVSYGEMIGCDNPDCPIEWFHFACVDLTTKPKGK

相同性解析による交差性が予測される生物種
Full Name inhibitor of growth family, member 5
Calculated molecular weight 28 kDa
Observed molecular weight 28-32 kDa
GenBank accession numberBC005370
Gene Symbol ING5
Gene ID (NCBI) 84289
RRIDAB_2280522
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WYH8
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

ING5, also named as p28ING5, belongs to the ING family. It is a component of the HBO1 complex which has a histone H4-specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo. ING5 is a component of the MOZ/MORF complex which has a histone H3 acetyltransferase activity. Through chromatin acetylation it may regulate DNA replication and may function as a transcriptional coactivator. (PMID:12750254) Altered ING5 expression and its shift into the cytoplasm may have an impact on the malignant transformation of colorectal epithelial cells and should be considered as a biomarker for colorectal carcinogenesis. (PMID:21193223)

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