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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ING1 Polyclonal antibody

ING1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 17921-1-AP

Synonyms

ING1, Inhibitor of growth protein 1, p24ING1c, p33, p33ING1, p33ING1b, p47, p47ING1a

Validation Data Gallery

Applications:
  • WB analysis of mouse liver using 17921-1-AP

    WB analysis of mouse liver using 17921-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 17921-1-AP (ING1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse liver using 17921-1-AP

    WB analysis of mouse liver using 17921-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 17921-1-AP (ING1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of A375 using 17921-1-AP

    WB analysis of A375 using 17921-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 17921-1-AP (ING1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inmouse liver tissue, A375 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Sample-dependent, check data in validation data gallery

Product Information

17921-1-AP targets ING1 in WB, ELISA applications and shows reactivity with mouse, human samples.

Tested Reactivity mouse, human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ING1 fusion protein Ag12338 相同性解析による交差性が予測される生物種
Full Name inhibitor of growth family, member 1
Calculated molecular weight279aa,32 kDa; 422aa,47 kDa
Observed molecular weight 47 kDa
GenBank accession numberBC093942
Gene symbol ING1
Gene ID (NCBI) 3621
RRIDAB_2878467
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ING1, also inhibitor of growth protein 1, is a 422 amino acid protein, which contains 1 PHD-type zinc finger and belongs to the ING family. ING1 localizes in the nucleus and interacts with TP53. It exsits as varous isoforms and isoform 1 is a 47 kDa protein. Isoform 2 is a 33 kDa protein and expressed in all normal tissues, as well as in all breast cancer and melanoma cell lines examined. ING1 cooperates with p53/TP53 in the negative regulatory pathway of cell growth by modulating p53-dependent transcriptional activation. It is implicated as a tumor suppressor gene.

Protocols

Product Specific Protocols
WB protocol for ING1 antibody 17921-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols