Validation Data Gallery
Tested Applications
| Positive WB detected in | A375 cells, mouse liver tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17921-1-AP targets ING1 in WB, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag12338 Product name: Recombinant human ING1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-279 aa of BC093942 Sequence: MLSPANGEQLHLVNYVEDYLDSIESLPFDLQRNVSLMREIDAKYQEILKELDECYERFSRETDGAQKRRMLHCVQRALIRSQELGDEKIQIVSQMVELVENRTRQVDSHVELFEAQQELGDTAGNSGKAGADRPKGEAAAQADKPNSKRSRRQRNNENRENASSNHDHDDGASGTPKEKKAKTSKKKKRSKAKAEREASPADLPIDPNEPTYCLCNQVSYGEMIGCDNDECPIEWFHFSCVGLNHKPKGKWYCPKCRGENEKTMDKALEKSKKERAYNR 相同性解析による交差性が予測される生物種 |
| Full Name | inhibitor of growth family, member 1 |
| Calculated molecular weight | 279aa,32 kDa; 422aa,47 kDa |
| Observed molecular weight | 24 kDa, 33 kDa and 47 kDa |
| GenBank accession number | BC093942 |
| Gene Symbol | ING1 |
| Gene ID (NCBI) | 3621 |
| RRID | AB_2878467 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UK53 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ING1 (Inhibitor of Growth 1) is a tumor suppressor gene located at human chromosome 13q33-34. It encodes multiple protein isoforms generated by alternative splicing and differential promoter usage, including p47ING1a (~47 kDa), p33ING1b (~33 kDa), p24ING1c (~24 kDa), and p27ING1d (~27 kDa) (PMID: 23412501). Among these, p33ING1b is the most abundantly expressed and best-characterized isoform in human cells (PMID: 41617915). All ING1 isoforms share a conserved C-terminal region containing a plant homeodomain (PHD) finger that recognizes the H3K4Me3 histone mark, enabling recruitment of histone deacetylase complexes to regulate chromatin structure and gene transcription. ING1 cooperates with p53 to induce cell cycle arrest and apoptosis, and its dysregulation is implicated in various cancers (PMID: 23255913).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ING1 antibody 17921-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |