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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using INADL Monoclonal antibody (67833-1-Ig)

    WB analysis using 67833-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67833-1-Ig (INADL antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using INADL Monoclonal antibody (67833-1-Ig)

    IF Staining of MCF-7 using 67833-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using INADL antibody (67833-1-Ig, Clone: 3C6A4 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

INADL Monoclonal antibody

INADL Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67833-1-Ig
Clone No.3C6A4

Host / Isotype

Mouse / IgG1

Reactivity

human, rat

Applications

WB, IF/ICC, ELISA

Synonyms

PATJ, Pals1-associated tight junction protein, InaD-like protein, hINADL, CIPP

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inLNCaP cells, HeLa cells, Jurkat cells, HEK-293 cells, K 562 cells, PC-12 cells
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67833-1-Ig targets INADL in WB, IF/ICC, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen INADL fusion protein Ag15953 相同性解析による交差性が予測される生物種
Full Name InaD-like (Drosophila)
Calculated molecular weight 1801 aa, 196 kDa
Observed molecular weight196 kDa
GenBank accession numberBC142661
Gene Symbol INADL
Gene ID (NCBI) 10207
RRIDAB_2918595
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ8NI35
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for INADL antibody 67833-1-IgDownload protocol
IF protocol for INADL antibody 67833-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols