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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human skin cancer tissue using IMUP Recombinant antibody (84148-6-RR)

    IHC staining of human skin cancer using 84148-6-RR (same clone as 84148-6-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 84148-6-RR (IMUP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84148-6-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using IMUP Recombinant antibody (84148-6-RR)

    IHC staining of human skin cancer using 84148-6-RR (same clone as 84148-6-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 84148-6-RR (IMUP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84148-6-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using IMUP Recombinant antibody (84148-6-RR)

    IF Staining of A431 using 84148-6-RR (same clone as 84148-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using C19orf33 antibody (84148-6-RR, Clone: 241220B4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84148-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84148-6-RR

    Affinity and Kinetic Characterization of 84148-6-RR

    Biolayer interferometry (BLl) kinetic assays of 84148-6-RR against Human IMUP were performed. The affinity constant is 10.3 nM.

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IMUP Recombinant antibody, PBS Only

IMUP Uni-rAbTM Recombinant Antibody for IHC, IF/ICC, Indirect ELISA
Cat No. 84148-6-PBS
Clone No.241220B4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, Indirect ELISA

Synonyms

Immortalization up-regulated protein, Hepatocyte growth factor activator inhibitor type 2-related small protein, HAI-2-related small protein, H2RSP, C19orf33

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84148-6-PBS targets IMUP in IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen IMUP fusion protein Ag22218 相同性解析による交差性が予測される生物種
Full Name chromosome 19 open reading frame 33
Calculated molecular weight 106 aa, 11 kDa
GenBank accession numberBC060319
Gene Symbol IMUP
Gene ID (NCBI) 64073
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9GZP8
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Immortalization up-regulated protein (IMUP), also known as C19orf33 or H2RSP, was first identified in SV40-immortalized fibroblasts and includes two isoforms, IMUP-1 and IMUP-2 (PMID: 14981917; 35142956). It is found primarily in the nucleus (PMID: 11606055). IMUP is reportedly upregulated in many cancer cells and tissues and its overexpression is associated with tumorigenicity and cell-cycle acceleration (PMID: 16962562; 35142956; 22886722). The apparent molecular determined by SDS-PAGE might be due to post-translational modifications or anomalous migration behavior (PMID: 11080599).