Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis of HeLa using 22092-1-AP
WB result of IMPDH1 antibody (22092-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-IMPDH1 transfected HeLa cells.
WB analysis using 22092-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 22092-1-AP (IMPDH1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 22092-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 22092-1-AP (IMPDH1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of mouse spleen using 22092-1-AP
mouse spleen tissue were subjected to SDS PAGE followed by western blot with 22092-1-AP (IMPDH1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 22092-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 22092-1-AP (IMPDH1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 22092-1-AP
IP result of anti-IMPDH1 (IP:22092-1-AP, 4ug; Detection:22092-1-AP 1:2000) with HeLa cells lysate 3200ug.
IHC staining of human ovary tumor using 22092-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor slide using 22092-1-AP (IMPDH1 Antibody) at dilution of 1:50.
IHC staining of human ovary tumor using 22092-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor slide using 22092-1-AP (IMPDH1 Antibody) at dilution of 1:50.
IHC staining of human tonsillitis using 22092-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 22092-1-AP (IMPDH1 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human tonsillitis using 22092-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 22092-1-AP (IMPDH1 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human tonsillitis using 22092-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis slide using 22092-1-AP (IMPDH1 Antibody) at dilution of 1:50.
IHC staining of human tonsillitis using 22092-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis slide using 22092-1-AP (IMPDH1 Antibody) at dilution of 1:50.
IF Staining of HeLa using 22092-1-AP
Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 22092-1-AP (IMPDH1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

IMPDH1 Polyclonal antibody

IMPDH1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 22092-1-AP

Publications(25)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

EC:1.1.1.205, IMP dehydrogenase 1, IMPD 1, IMPD1, IMPDH 1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (25)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, mouse spleen tissue, A431 cells, K-562 cells, Raji cells, rat spleen tissue
Positive IP detected in	HeLa cells
Positive IHC detected in	human tonsillitis tissue, human ovary tumor tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:800-1:3200
Immunofluorescence (IF)/ICC	IF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 16 publications below
IHC	See 4 publications below
IF	See 6 publications below
IP	See 1 publications below

Product Information

22092-1-AP targets IMPDH1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag17297 Product name: Recombinant human IMPDH1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 170-235 aa of BC033622 Sequence: LSPSHTVGDVLEAKMRHGFSGIPITETGTMGSKLVGIVTSRDIDFLAEKDHTTLLSEVMTPRIELV 相同性解析による交差性が予測される生物種
Full Name	IMP (inosine monophosphate) dehydrogenase 1
Calculated molecular weight	563 aa, 60 kDa
Observed molecular weight	53-60 kDa
GenBank accession number	BC033622
Gene Symbol	IMPDH1
Gene ID (NCBI)	3614
RRID	AB_2878992
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P20839
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

IMPDH1(Inosine-5'-monophosphate dehydrogenase 1) is also named as IMPD1, IMP dehydrogenase 1 and belongs to the IMPDH/GMPR family. It catalyzes the rate limiting step of de novo guanine synthesis and an important target for the development of drugs with both chemotherapeutic and immunosuppressive activity. It may also have a role in the development of malignancy and the growth progression of some tumors. This protein has some isoforms produced by alternative splicing with the molecular mass of 53-65 kDa. This antibody may has cross reaction with IMPDH2 due to their high homology.

Protocols

Product Specific Protocols
IF protocol for IMPDH1 antibody 22092-1-AP	Download protocol
IHC protocol for IMPDH1 antibody 22092-1-AP	Download protocol
IP protocol for IMPDH1 antibody 22092-1-AP	Download protocol
WB protocol for IMPDH1 antibody 22092-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse,human	WB	Cell De novo and salvage purine synthesis pathways across tissues and tumors Authors - Diem H Tran View Article
		Nat Commun Targeting spermine metabolism to overcome immunotherapy resistance in pancreatic cancer Authors - Hanshen Yang View Article
human	WB	Mol Cell ERK2 Phosphorylates PFAS to Mediate Posttranslational Control of De Novo Purine Synthesis. Authors - Eunus S Ali View Article
		Mol Cell Accumulation of succinate suppresses de novo purine synthesis through succinylation-mediated control of the mitochondrial folate cycle. Authors - Mushtaq A. Nengroo View Article
mouse	WB	Int J Biol Sci LncRNA UCA1 Participates in De Novo Synthesis of Guanine Nucleotides in Bladder Cancer by Recruiting TWIST1 to Increase IMPDH1/2 Authors - Shan-Shan Liu View Article
human	WB,IP,IHC,IF	Mol Ther IMPDH1/YB-1 Positive Feedback Loop Assembles Cytoophidia and Represents a Therapeutic Target in Metastatic Tumors. Authors - Hailong Ruan KD Validated View Article

Validation Data Gallery

WB analysis of HeLa using 22092-1-AP

WB analysis using 22092-1-AP

WB analysis of HeLa using 22092-1-AP

WB analysis of mouse spleen using 22092-1-AP

WB analysis of A431 using 22092-1-AP

IP experiment of HeLa using 22092-1-AP

IHC staining of human ovary tumor using 22092-1-AP

IHC staining of human ovary tumor using 22092-1-AP

IHC staining of human tonsillitis using 22092-1-AP

IHC staining of human tonsillitis using 22092-1-AP

IHC staining of human tonsillitis using 22092-1-AP

IHC staining of human tonsillitis using 22092-1-AP

IF Staining of HeLa using 22092-1-AP

IMPDH1 Polyclonal antibody

IMPDH1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

De novo and salvage purine synthesis pathways across tissues and tumors

Targeting spermine metabolism to overcome immunotherapy resistance in pancreatic cancer

ERK2 Phosphorylates PFAS to Mediate Posttranslational Control of De Novo Purine Synthesis.

Accumulation of succinate suppresses de novo purine synthesis through succinylation-mediated control of the mitochondrial folate cycle.

LncRNA UCA1 Participates in De Novo Synthesis of Guanine Nucleotides in Bladder Cancer by Recruiting TWIST1 to Increase IMPDH1/2

IMPDH1/YB-1 Positive Feedback Loop Assembles Cytoophidia and Represents a Therapeutic Target in Metastatic Tumors.

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