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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IMPACT Polyclonal antibody (16528-1-AP)

    WB analysis using 16528-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16528-1-AP (IMPACT antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of TT cells using IMPACT Polyclonal antibody (16528-1-AP)

    WB analysis of TT using 16528-1-AP

    TT cells were subjected to SDS PAGE followed by western blot with 16528-1-AP (IMPACT antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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IMPACT Polyclonal antibody

IMPACT Polyclonal Antibody for WB, ELISA
Cat No. 16528-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

IMPACT, Protein IMPACT

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, TT cells, HL-60 cells, LNCaP cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16528-1-AP targets IMPACT in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IMPACT fusion protein Ag9776 相同性解析による交差性が予測される生物種
Full Name Impact homolog (mouse)
Calculated molecular weight 320 aa, 36 kDa
Observed molecular weight40 kDa
GenBank accession numberBC034016
Gene Symbol IMPACT
Gene ID (NCBI) 55364
RRIDAB_3669247
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9P2X3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for IMPACT antibody 16528-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols