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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IMPACT Polyclonal antibody (16528-1-AP)

    WB analysis using 16528-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16528-1-AP (IMPACT antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of TT cells using IMPACT Polyclonal antibody (16528-1-AP)

    WB analysis of TT using 16528-1-AP

    TT cells were subjected to SDS PAGE followed by western blot with 16528-1-AP (IMPACT antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

IMPACT Polyclonal antibody

IMPACT Polyclonal Antibody for WB, ELISA
Cat No. 16528-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

Protein IMPACT

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, TT cells, HL-60 cells, LNCaP cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16528-1-AP targets IMPACT in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag9776

Product name: Recombinant human IMPACT protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-320 aa of BC034016

Sequence: MAEGDAGSDQRQNEEIEAMAAIYGEEWCVIDDCAKIFCIRISDDIDDPKWTLCLQVMLPNEYPGTAPPIYQLNAPWLKGQERADLSNSLEEIYIQNIGESILYLWVEKIRDVLIQKSQMTEPGPDVKKKTEEEDVECEDDLILACQPESSVKALDFDISETRTEVEVEELPPIDHGIPITDRRSTFQAHLAPVVCPKQVKMVLSKLYENKKIASATHNIYAYRIYCEDKQTFLQDCEDDGETAAGGRLLHLMEILNVKNVMVVVSRWYGGILLGPDRFKHINNCARNILVEKNYTNSPEESSKALGKNKKVRKDKKRNEH

相同性解析による交差性が予測される生物種
Full Name Impact homolog (mouse)
Calculated molecular weight 320 aa, 36 kDa
Observed molecular weight40 kDa
GenBank accession numberBC034016
Gene Symbol IMPACT
Gene ID (NCBI) 55364
RRIDAB_3669247
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9P2X3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Mouse Impact is a paternally expressed imprinted gene, encoding a highly conserved protein whose exact biological function remains under investigation. It is located in a syntenic region on mouse chromosome 18, corresponding to human chromosome.

Protocols

Product Specific Protocols
WB protocol for IMPACT antibody 16528-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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