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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IMPA1 Polyclonal antibody (16593-1-AP)

    WB analysis using 16593-1-AP (same clone as 16593-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16593-1-AP (IMPA1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16593-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using IMPA1 Polyclonal antibody (16593-1-AP)

    WB analysis using 16593-1-AP (same clone as 16593-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16593-1-AP (IMPA1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16593-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human kidney tissue using IMPA1 Polyclonal antibody (16593-1-AP)

    WB analysis of human kidney using 16593-1-AP (same clone as 16593-1-PBS)

    human kidney tissue were subjected to SDS PAGE followed by western blot with 16593-1-AP (IMPA1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16593-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using IMPA1 Polyclonal antibody (16593-1-AP)

    WB analysis of human brain using 16593-1-AP (same clone as 16593-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 16593-1-AP (IMPA1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16593-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using IMPA1 Polyclonal antibody (16593-1-AP)

    IF Staining of HepG2 using 16593-1-AP (same clone as 16593-1-PBS)

    Immunofluorescent analysis of HepG2 cells, using IMPA1 antibody 16593-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 16593-1-PBS in a different storage buffer formulation.

IMPA1 Polyclonal antibody, PBS Only

IMPA1 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 16593-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

D-galactose 1-phosphate phosphatase, EC:3.1.3.25, EC:3.1.3.94, IMP 1, IMP1

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16593-1-PBS targets IMPA1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag9632

Product name: Recombinant human IMPA1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-277 aa of BC008381

Sequence: MADPWQECMDYAVTLARQAGEVVCEAIKNEMNVMLKSSPVDLVTATDQKVEKMLISSIKEKYPSHSFIGEESVAAGEKSILTDNPTWIIDPIDGTTNFVHRFPFVAVSIGFAVNKKIEFGVVYSCVEGKMYTARKGKGAFCNGQKLQVSQQEDITKSLLVTELGSSRTPETVRMVLSNMEKLFCIPVHGIRSVGTAAVNMCLVATGGADAYYEMGIHCWDVAGAGIIVTEAGGVLMDVTGGPFDLMSRRVIAANNRILAERIAKEIQVIPLQRDDED

相同性解析による交差性が予測される生物種
Full Name inositol(myo)-1(or 4)-monophosphatase 1
Calculated molecular weight 277 aa, 30 kDa
Observed molecular weight 30 kDa
GenBank accession numberBC008381
Gene Symbol IMPA1
Gene ID (NCBI) 3612
RRIDAB_2127216
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP29218
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

IMPA1 is an enzyme that dephosphorylates myo-inositol monophosphate to generate free myo-inositol, a precursor of phosphatidylinositol, and is therefore an important modulator of intracellular signal transduction via the production of the second messengers myoinositol 1,4,5-trisphosphate and diacylglycerol.

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