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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IMP5 Polyclonal antibody

IMP5 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12664-1-AP

Synonyms

IMP 5, IMP5, intramembrane protease 5, SPP like 2C, SPPL2c

Validation Data Gallery

Applications:
  • WB analysis of HEK-293 using 12664-1-AP

    WB analysis of HEK-293 using 12664-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 12664-1-AP (IMP5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 12664-1-AP

    WB analysis of HeLa using 12664-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12664-1-AP (IMP5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of human liver using 12664-1-AP

    WB analysis of human liver using 12664-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 12664-1-AP (IMP5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of HEK-293 using 12664-1-AP

    IP experiment of HEK-293 using 12664-1-AP

    IP result of anti-IMP5 (IP:12664-1-AP, 4ug; Detection:12664-1-AP 1:500) with HEK-293 cells lysate 3600ug.

  • IHC staining of human liver cancer using 12664-1-AP

    IHC staining of human liver cancer using 12664-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12664-1-AP (IMP5 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human liver cancer using 12664-1-AP

    IHC staining of human liver cancer using 12664-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12664-1-AP (IMP5 Antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HEK-293 using 12664-1-AP

    IF Staining of HEK-293 using 12664-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 12664-1-AP (IMP5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, human liver tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

12664-1-AP targets IMP5 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IMP5 fusion protein Ag3345 相同性解析による交差性が予測される生物種
Full Name intramembrane protease 5
Calculated molecular weight 684 aa, 74 kDa
Observed molecular weight 74 kDa
GenBank accession numberBC025401
Gene symbol IMP5
Gene ID (NCBI) 162540
RRIDAB_2296163
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for IMP5 antibody 12664-1-APDownload protocol
IHC protocol for IMP5 antibody 12664-1-APDownload protocol
IF protocol for IMP5 antibody 12664-1-APDownload protocol
IP protocol for IMP5 antibody 12664-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nature

MIR-NATs repress MAPT translation and aid proteostasis in neurodegeneration.

Authors - Roberto Simone
View Article