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  • Featured Product
  • KD/KO Validated

IMP3 Polyclonal antibody

IMP3 Polyclonal Antibody for WB, IP, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12750-1-AP

Synonyms

BRMS2, C15orf12, IMP3, MRPS4, U3 snoRNP protein IMP3

Validation Data Gallery

Applications:
  • WB analysis using 12750-1-AP

    WB analysis using 12750-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12750-1-AP (IMP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 12750-1-AP

    WB analysis of HeLa using 12750-1-AP

    WB result of IMP3 antibody (12750-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-IMP3 transfected HeLa cells.

  • WB analysis of MCF-7 using 12750-1-AP

    WB analysis of MCF-7 using 12750-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 12750-1-AP (IMP3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 12750-1-AP

    IP experiment of HeLa using 12750-1-AP

    IP result of anti-IMP3 (IP:12750-1-AP, 3ug; Detection:12750-1-AP 1:500) with HeLa cells lysate 1200ug.

  • IF Staining of HeLa using 12750-1-AP

    IF Staining of HeLa using 12750-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 12750-1-AP (IMP3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, MCF-7 cells
Positive IP detected inHeLa cells
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

12750-1-AP targets IMP3 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IMP3 fusion protein Ag3477 相同性解析による交差性が予測される生物種
Full Name IMP3, U3 small nucleolar ribonucleoprotein, homolog (yeast)
Calculated molecular weight 184 aa, 22 kDa
Observed molecular weight 22 kDa
GenBank accession numberBC020557
Gene symbol IMP3
Gene ID (NCBI) 55272
RRIDAB_2122784
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IMP U3 small nucleolar ribonucleoprotein 3 (IMP3, BRMS2), is a component of U3 small nucleolar ribonucleoprotein complex (U3 snoRNP), has been identified as a participant in pre-rRNA processing for nearly twenty years.

Protocols

Product Specific Protocols
WB protocol for IMP3 antibody 12750-1-APDownload protocol
IF protocol for IMP3 antibody 12750-1-APDownload protocol
IP protocol for IMP3 antibody 12750-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IHC

Oncotarget

IMP2/IGF2BP2 expression, but not IMP1 and IMP3, predicts poor outcome in patients and high tumor growth rate in xenograft models of gallbladder cancer.

Authors - Sonja M Kessler
View Article