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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IMMT Recombinant monoclonal antibody (88213-2-RR)

    WB analysis using 88213-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 88213-2-RR (IMMT antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using IMMT Recombinant monoclonal antibody (88213-2-RR)

    IF Staining of HepG2 using 88213-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using IMMT antibody (88213-2-RR, Clone: 260293A12 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

IMMT Recombinant monoclonal antibody

IMMT Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 88213-2-RR
Clone No.260293A12

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

Cell proliferation-inducing gene 4/52 protein, HMP, MIC60, MICOS complex subunit MIC60, MINOS2

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, HEK-293 cells, Raji cells, A-673 cells, mouse brain tissue
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

88213-2-RR targets IMMT in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag0102

Product name: Recombinant human IMMT protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 4-297 aa of BC002412

Sequence: ACQLSGVTAAAQSCLCGKFVLRPLRPCRRYSTSGSSGLTTGKIAGAGLLFVGGGIGGTILYAKWDSHFRESVEKTIPYSDKLFEMVLGPAAYNVPLPKKSIQSGPLKISSVSEVMKESKQPASQLQKQKGDTPASATAPTEAAQIISAAGDTLSVPAPAVQPEESLKTDHPEIGEGKPTPALSEEASSSSIRERPPEEVAARLAQQEKQEQVKIESLAKSLEDALRQTASVTLQAIAAQNAAVQAVNAHSNILKAAMDNSEIAGEKKSAQWRTVEGALKERRKAVDEAADALLK

相同性解析による交差性が予測される生物種
Full Name inner membrane protein, mitochondrial (mitofilin)
Calculated molecular weight 90 kDa
Observed molecular weight 80-90 kDa
GenBank accession numberBC002412
Gene Symbol IMMT
Gene ID (NCBI) 10989
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ16891
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IMMT (also known as mitofilin) is an inner mitochondrial membrane protein that is preferentially expressed in heart tissue and is commonly used as the marker for mitochondria. Mitofilin is known to be a critical organizer of mitochondrial cristae morphology and is indispensable for normal mitochondrial function.

Protocols

Product Specific Protocols
IF protocol for IMMT antibody 88213-2-RRDownload protocol
WB protocol for IMMT antibody 88213-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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