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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ILVBL Polyclonal antibody (11220-1-AP)

    WB analysis using 11220-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11220-1-AP (ILVBL antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human thyroid gland tissue using ILVBL Polyclonal antibody (11220-1-AP)

    WB analysis of human thyroid gland using 11220-1-AP

    human thyroid gland tissue were subjected to SDS PAGE followed by western blot with 11220-1-AP (ILVBL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using ILVBL Polyclonal antibody (11220-1-AP)

    IHC staining of human colon cancer using 11220-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11220-1-AP (ILVBL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using ILVBL Polyclonal antibody (11220-1-AP)

    IHC staining of human colon cancer using 11220-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11220-1-AP (ILVBL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using ILVBL Polyclonal antibody (11220-1-AP)

    IHC staining of human colon cancer using 11220-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11220-1-AP (ILVBL antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ILVBL Polyclonal antibody (11220-1-AP)

    IF Staining of HeLa using 11220-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ILVBL antibody (11220-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

ILVBL Polyclonal antibody

ILVBL Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 11220-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

HACL2, EC:4.1.2.-, AHAS, Acetolactate synthase-like protein, 2-hydroxyacyl-CoA lyase 2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA549 cells, human thyroid gland tissue, HepG2 cells, HeLa cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:800-1:3200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11220-1-AP targets ILVBL in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag1729

Product name: Recombinant human ILVBL protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 284-632 aa of BC011761

Sequence: KRPLMVLGSQALLTPTSADKLRAAVETLGVPCFLGGMARGLLGRNHPLHIRENRSAALKKADVIVLAGTVCDFRLSYGRVLSHSSKIIIVDRNREEMLLNSDIFWKPQEAVQGDVGSFVLKLVEGLQGQTWAPDWVEELREADRQKEQTFREKAAMPVAQHLNPVQVLQLVEETLPDNSILVVDGGDFVGTAAHLVQPRGPLRWLDPGAFGTLGVGAGFALGAKLCRPDAEVWCLFGDGAFGYSLIEFDTFVRHKIPVMALVGNDAGWTQISREQVPSLGSNVACGLAYTDYHKAAMGLGARGLLLSRENEDQVVKVLHDAQQQCRDGHPVVVNILIGRTDFRDGSIAV

相同性解析による交差性が予測される生物種
Full Name ilvB (bacterial acetolactate synthase)-like
Calculated molecular weight 68 kDa
Observed molecular weight 60-65 kDa
GenBank accession numberBC011761
Gene Symbol ILVBL
Gene ID (NCBI) 10994
RRIDAB_2127187
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDA1L0T0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ILVBL antibody 11220-1-APDownload protocol
IHC protocol for ILVBL antibody 11220-1-APDownload protocol
IF protocol for ILVBL antibody 11220-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols