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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ILVBL Polyclonal antibody

ILVBL Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11220-1-AP

Synonyms

209L8, AHAS, ILV2H, IlvB like protein, ILVBL

Validation Data Gallery

Applications:
  • WB analysis using 11220-1-AP

    WB analysis using 11220-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11220-1-AP (ILVBL antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis of human thyroid gland using 11220-1-AP

    WB analysis of human thyroid gland using 11220-1-AP

    human thyroid gland tissue were subjected to SDS PAGE followed by western blot with 11220-1-AP (ILVBL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 11220-1-AP

    WB analysis of HepG2 using 11220-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 11220-1-AP (ILVBL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of A549 using 11220-1-AP

    WB analysis of A549 using 11220-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 11220-1-AP (ILVBL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 11220-1-AP

    WB analysis of HeLa using 11220-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 11220-1-AP (ILVBL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 11220-1-AP

    IHC staining of human colon cancer using 11220-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11220-1-AP (ILVBL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 11220-1-AP

    IHC staining of human colon cancer using 11220-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11220-1-AP (ILVBL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 11220-1-AP

    IF Staining of HeLa using 11220-1-AP

    Immunofluorescent analysis of Hela cells, using ILVBL antibody 11220-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive WB detected inA549 cells, human thyroid gland tissue, HepG2 cells, HeLa cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

11220-1-AP targets ILVBL in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ILVBL fusion protein Ag1729 相同性解析による交差性が予測される生物種
Full Name ilvB (bacterial acetolactate synthase)-like
Calculated molecular weight 68 kDa
Observed molecular weight 60-65 kDa
GenBank accession numberBC011761
Gene symbol ILVBL
Gene ID (NCBI) 10994
RRIDAB_2127187
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ILVBL antibody 11220-1-APDownload protocol
IHC protocol for ILVBL antibody 11220-1-APDownload protocol
IF protocol for ILVBL antibody 11220-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols