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Validation Data Gallery

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  • Western Blot (WB) analysis of human heart tissue using ILKAP Polyclonal antibody (16017-1-AP)

    WB analysis of human heart using 16017-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 16017-1-AP (ILKAP antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using ILKAP Polyclonal antibody (16017-1-AP)

    WB analysis of HepG2 using 16017-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 16017-1-AP (ILKAP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using ILKAP Polyclonal antibody (16017-1-AP)

    WB analysis of K-562 using 16017-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 16017-1-AP (ILKAP antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human heart tissue using ILKAP Polyclonal antibody (16017-1-AP)

    WB analysis of human heart using 16017-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 16017-1-AP (ILKAP antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using ILKAP Polyclonal antibody (16017-1-AP)

    IHC staining of human stomach cancer using 16017-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 16017-1-AP (ILKAP antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using ILKAP Polyclonal antibody (16017-1-AP)

    IHC staining of human stomach cancer using 16017-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 16017-1-AP (ILKAP antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ILKAP Polyclonal antibody

ILKAP Polyclonal Antibody for WB, IHC, ELISA
Cat No. 16017-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

ILKAP, PP2C DELTA

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman heart tissue, HepG2 cells, K-562 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16017-1-AP targets ILKAP in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag8826

Product name: Recombinant human ILKAP protein

Source: e coli.-derived, T-HIS

Tag: 6*His

Domain: 1-392 aa of BC006576

Sequence: MDLFGDLPEPERSPRPAAGKEAQKGPLLFDDLPPASSTDSGSGGPLLFDDLPPASSGDSGSLATSISQMVKTEGKGAKRKTSEEEKNGSEELVEKKVCKASSVIFGLKGYVAERKGEREEMQDAHVILNDITEECRPPSSLITRVSYFAVFDGHGGIRASKFAAQNLHQNLIRKFPKGDVISVEKTVKRCLLDTFKHTDEEFLKQASSQKPAWKDGSTATCVLAVDNILYIANLGDSRAILCRYNEESQKHAALSLSKEHNPTQYEERMRIQKAGGNVRDGRVLGVLEVSRSIGDGQYKRCGVTSVPDIRRCQLTPNDRFILLACDGLFKVFTPEEAVNFILSCLEDEKIQTREGKSAADARYEAACNRLANKAVQRGSADNVTVMVVRIGH

相同性解析による交差性が予測される生物種
Full Name integrin-linked kinase-associated serine/threonine phosphatase 2C
Calculated molecular weight 392 aa, 43 kDa
Observed molecular weight 43-45 kDa
GenBank accession numberBC006576
Gene Symbol ILKAP
Gene ID (NCBI) 80895
RRIDAB_2249188
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H0C8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IHC protocol for ILKAP antibody 16017-1-APDownload protocol
WB protocol for ILKAP antibody 16017-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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