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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IL-3RA Polyclonal antibody

IL-3RA Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13655-1-AP

Synonyms

CD123, hIL 3Ra, IL 3 receptor subunit alpha, IL 3R alpha, IL 3R subunit alpha, IL 3RA, IL3R, IL3RA, IL-3RA, IL3RAY, IL3RX, IL3RY

Validation Data Gallery

Applications:
  • WB analysis of HL-60 using 13655-1-AP

    WB analysis of HL-60 using 13655-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 13655-1-AP (IL3RA antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 13655-1-AP

    IHC staining of human tonsillitis using 13655-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13655-1-AP (IL3RA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 13655-1-AP

    IHC staining of human tonsillitis using 13655-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13655-1-AP (IL3RA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHL-60 cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

13655-1-AP targets IL-3RA in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IL-3RA fusion protein Ag4452 相同性解析による交差性が予測される生物種
Full Name interleukin 3 receptor, alpha (low affinity)
Calculated molecular weight 378 aa, 43 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC035407
Gene symbol IL3RA
Gene ID (NCBI) 3563
RRIDAB_10598015
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for IL-3RA antibody 13655-1-APDownload protocol
IHC protocol for IL-3RA antibody 13655-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols