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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IL23(IL12B+IL23A) Polyclonal antibody (32180-1-AP)

    WB analysis using 32180-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32180-1-AP (IL23(IL12B+IL23A) antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of Raji cells using IL23(IL12B+IL23A) Polyclonal antibody (32180-1-AP)

    IF Staining of Raji using 32180-1-AP

    Immunofluorescent analysis of (4% PFA) fixed Raji cells using IL23(IL12B+IL23A) antibody (32180-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

IL23(IL12B+IL23A) Polyclonal antibody

IL23(IL12B+IL23A) Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 32180-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

IL 23, IL23 (IL12B+IL23A), IL-23, IL23

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells, SW480 cells, U266 cells
Positive IF/ICC detected inRaji cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

32180-1-AP targets IL23(IL12B+IL23A) in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name IL23(IL12B+IL23A)
Calculated molecular weight54 kDa, 37 kDa, 21 kDa
Observed molecular weight54 kDa, 37 kDa
Gene Symbol
Gene ID (NCBI)
RRIDAB_3670216
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDP29460(IL12B)+linker+Q9NPF7(IL23A)
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IL-12 and IL-23 are heterodimeric cytokines with a common p40 subunit (PMID: 11114383). IL-12 is composed of the IL-12 p40 subunit linked to the IL-12 p35 subunit, and the heterodimer signals through the IL-12 receptor (IL-12R), which comprises the IL-12Rβ1 and IL-12Rβ2 subunits. IL-23 is composed of the IL-23 p19 subunit and the IL-12 p40 (IL-12/23p40) subunit, which signals through IL-23R and IL-12Rβ1 (PMID: 11114383; 26121196 ). The importance of IL-23 has been confirmed by clinical data, as monoclonal antibodies (MABs) targeting the p40 subunit (shared by IL-23 and IL-12), as well as p19 (specific for IL-23) were effective in therapy for psoriasis and psoriatic arthritis (PMID: 25754330; 18486740). This antibody can recognize the IL12B monomer (37 kDa) and the heterodimer (IL12B+IL23A, 54 kDa).

Protocols

Product Specific Protocols
WB protocol for IL23(IL12B+IL23A) antibody 32180-1-APDownload protocol
IF protocol for IL23(IL12B+IL23A) antibody 32180-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols