Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells |
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IF | See 1 publications below |
Product Information
18104-1-AP targets IL-20 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | IL-20 fusion protein Ag12447 相同性解析による交差性が予測される生物種 |
| Full Name | interleukin 20 |
| Calculated molecular weight | 406 aa, 44 kDa |
| GenBank accession number | BC074949 |
| Gene Symbol | IL-20 |
| Gene ID (NCBI) | 50604 |
| RRID | AB_2878501 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NYY1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Interleukin-20 (IL-20) is a protein belonging to the IL-10 family of cytokines. IL-20 receptor (IL-20R) and IL-20 are expressed in several normal tissue types, including the lung, skin, and kidney. IL-20 is a cytokine structurally related to interleukin 10. IL-20 is produced by activated keratinocytes and monocytes and transmits an intracellular signal through two distinct cell-surface receptor complexes on keratinocytes and other epithelial cells. IL-20 can stimulate STAT3 activation in keratinocytes. IL-20 regulates proliferation and differentiation of keratinocytes during inflammation, particularly inflammation associated with the skin. In addition, IL-20 also causes cell expansion of multipotential hematopoietic progenitor cells. IL-20 is highly associated with potent inflammatory diseases such as psoriasis, contact hypersensitivity, rheumatoid arthritis, and atherosclerosis.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for IL-20 antibody 18104-1-AP | Download protocol |
| IHC protocol for IL-20 antibody 18104-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Immunology IL-20 is involved in obesity by modulation of adipogenesis and macrophage dysregulation. |