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Validation Data Gallery

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  • Sandwich ELISA standard curve of MP01289-3

    Sandwich ELISA standard curve of MP01289-3

    Sandwich ELISA standard curve of MP01289-3, Human IL-1 alpha Recombinant Matched Antibody Pair - PBS only. 84411-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard HZ-1320. 84411-3-PBS was HRP conjugated as the detection antibody. Range: 9.77-313 pg/mL.

  • Sample test of MP01289-3

    Sample test of MP01289-3

    Human peripheral blood mononuclear cells (PBMC) were cultured unstimulated or stimulated with 10 μg/mL PHA for 5 days. The mean IL-1 alpha concentration was determined to be 2.1 pg/mL in unstimulated PBMC supernatant, 880.0 pg/mL in PHA stimulated PBMC supernatant.

  • Sample test of MP01289-3

    Sample test of MP01289-3

    The mean IL-1 alpha concentration was determined to be 271.0 ng/mL in mouse liver tissue extract based on a 3.7 mg/mL extract load.

  • Sample test of MP01289-3

    Sample test of MP01289-3

    Serum of five individual healthy human donors was measured. The IL-1 alpha concentration of detected samples was determined to be 5.0 pg/mL with a range of 1.8-8.2 pg/mL.

IL1A Recombinant monoclonal antibody, PBS Only (Capture)

IL1A Uni-rAb® Recombinant Antibody for Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 84411-2-PBS
Clone No.241752E8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

IL 1 alpha, IL-1 alpha, Hematopoietin-1, IL 1A, IL1 ALPHA

Formulation:  PBS Only
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84411-2-PBS targets IL1A as part of a matched antibody pair:

MP01289-3: 84411-2-PBS capture and 84411-3-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

Recombinant protein

相同性解析による交差性が予測される生物種
Full Name interleukin 1, alpha
Calculated molecular weight 271 aa, 31 kDa
GenBank accession numberBC013142
Gene Symbol IL-1 alpha
Gene ID (NCBI) 3552
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP01583
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.