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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using IL-10RB Polyclonal antibody (19387-1-AP)

    WB analysis of Jurkat using 19387-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 19387-1-AP (IL10RB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using IL-10RB Polyclonal antibody (19387-1-AP)

    WB analysis of Jurkat using 19387-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 19387-1-AP (IL10RB antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using IL-10RB Polyclonal antibody (19387-1-AP)

    WB analysis of K-562 using 19387-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 19387-1-AP (IL10RB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using IL-10RB Polyclonal antibody (19387-1-AP)

    WB analysis of HeLa using 19387-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 19387-1-AP (IL10RB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

This product has been discontinued. We recommend 15102-1-AP as an alternative.
For more information, please contact us.

Tested Applications

Positive WB detected inJurkat cells, HeLa cells, K-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19387-1-AP targets IL-10RB in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IL-10RB fusion protein Ag7468 相同性解析による交差性が予測される生物種
Full Name interleukin 10 receptor, beta
Calculated molecular weight 37 kDa
Observed molecular weight 37-41 kDa
GenBank accession numberBC001903
Gene Symbol IL10RB
Gene ID (NCBI) 3588
RRIDAB_10643371
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ08334
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for IL-10RB antibody 19387-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols