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Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using CoraLite® Plus 488 Anti-Human IL-7 (1C11B11) (CL488-65281)

    FC experiment of human PBMCs using CL488-65281

    1X10^6 LPS and Brefeldin A treated human PBMCs were intracellularly stained with 0.2 ug CoraLite® Plus 488 Anti-Human IL-7 (CL488-65281, Clone:1C11B11) or Mouse IgG1 Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Monocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using CoraLite® Plus 488 Anti-Human IL-7 (1C11B11) (CL488-65281)

    FC experiment of human PBMCs using CL488-65281

    1X10^6 LPS and Brefeldin A treated human PBMCs were intracellularly stained with 0.2 ug CoraLite® Plus 488 Anti-Human IL-7 (CL488-65281, Clone:1C11B11) (red) or Mouse IgG1 Isotype Control (blue), or unactivated and Brefeldin A treated human PBMCs were intracellularly stained with 0.2 ug CoraLite® Plus 488 Anti-Human IL-7 (CL488-65281, Clone:1C11B11) (black, dashed line). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Monocytes were gated.

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CoraLite® Plus 488 Anti-Human IL-7 (1C11B11)

IL-7 Monoclonal Antibody for FC (Intra)
Cat No. CL488-65281
Clone No.1C11B11

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

FC (Intra)

Synonyms

IL7, IL-7, interleukin 7, Interleukin-7, 1C11B11, IL 7

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration:  100 μg, 0.2 mg/mL
100 μg, 0.2 mg/mL

¥75,000/ 100 μg

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive FC (Intra) detected inLPS and Brefeldin A treated human PBMCs
Positive FC detected inLPS and Brefeldin A treated human PBMCs

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.2 ug per 10^6 cells in 100 μl suspension
Flow Cytometry (FC)FC :
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-65281 targets IL-7 in FC (Intra) applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen IL-7 fusion protein HZ-1281 相同性解析による交差性が予測される生物種
Full Name interleukin 7
Calculated molecular weight 20 kDa
GenBank accession numberBC047698
Gene Symbol IL-7
Gene ID (NCBI) 3574
RRIDAB_3084193
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA{{ptg:BufferTemp}}7.3
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Interleukin-7 (IL-7) is a cytokine involved in B and T cell development. It plays an active role in the development, survival, maintaining and restoring homeostasis of mature T lymphocytes and is a key regulator of the commitment, survival, proliferation and maturation of B cells during development. Furthermore, IL-7 can improve the antiviral function and expansion of natural killer (NK) cells and regulate the development and differentiation of dendritic cells. IL-7 has also been reported as a regulator of the development of central nervous system and myogenesis and skeletal muscle cell migration. (PMID: 29663382; 29655570; 29449560)

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 IL-7 antibody CL488-65281Download protocol
Standard Protocols
Click here to view our Standard Protocols