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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IL-6 Monoclonal antibody (66146-1-Ig)

    WB analysis using 66146-1-Ig (same clone as 66146-1-PBS)

    Non-treated and protein transport inhibitor treated U-87 MG cells were subjected to SDS PAGE followed by western blot with 66146-1-Ig (IL-6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66146-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using IL-6 Monoclonal antibody (66146-1-Ig)

    WB analysis using 66146-1-Ig (same clone as 66146-1-PBS)

    Non-treated, LPS and protein transport inhibitor treated HUVEC cells were subjected to SDS PAGE followed by western blot with 66146-1-Ig (IL-6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66146-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Recombinant protein using IL-6 Monoclonal antibody (66146-1-Ig)

    WB analysis of Recombinant protein using 66146-1-Ig (same clone as 66146-1-PBS)

    Recombinant protein were subjected to SDS PAGE followed by western blot with 66146-1-Ig (IL-6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66146-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using IL-6 Monoclonal antibody (66146-1-Ig)

    WB analysis of Jurkat using 66146-1-Ig (same clone as 66146-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66146-1-Ig (IL-6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66146-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HUVEC cells using IL-6 Monoclonal antibody (66146-1-Ig)

    IF Staining of HUVEC using 66146-1-Ig (same clone as 66146-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed LPS and Brefeldin A treated HUVEC cells using IL-6 antibody (66146-1-Ig, Clone: 1A3B4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66146-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

IL-6 Monoclonal antibody, PBS Only

IL-6 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 66146-1-PBS
Clone No.1A3B4

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

IL6, Interferon beta-2, IL 6, IFN-beta-2, B-cell stimulatory factor 2

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66146-1-PBS targets IL-6 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag10646

Product name: Recombinant human IL-6 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-212 aa of BC015511

Sequence: MNSFSTSAFGPVAFSLGLLLVLPAAFPAPVPPGEDSKDVAAPHRQPLTSSERIDKQIRYILDGISALRKETCNKSNMCESSKEALAENNLNLPKMAEKDGCFQSGFNEETCLVKIITGLLEFEVYLEYLQNRFESSEEQARAVQMSTKVLIQFLQKKAKNLDAITTPDPTTNASLLTKLQAQNQWLQDMTTHLILRSFKEFLQSSLRALRQM

相同性解析による交差性が予測される生物種
Full Name interleukin 6 (interferon, beta 2)
Calculated molecular weight 212 aa, 24 kDa
Observed molecular weight24 kDa
GenBank accession numberBC015511
Gene Symbol IL6
Gene ID (NCBI) 3569
ENSEMBL Gene IDENSG00000136244
RRIDAB_2881543
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP05231
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Interleukin-6 (IL-6) is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine. IL-6 protein is secreted by a variety of cell types including T cells and macrophages as phosphorylated and variably glycosylated molecule. IL-6 plays an essential role in the final differentiation of B-cells into Ig-secreting cells involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. IL-6 is also considered a myokine, a cytokine produced from muscle, and is elevated in response to muscle contraction. IL-6 has been shown to interact with interleukin-6 receptor and glycoprotein 130. Additionally, IL-6 is involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined an essential role in directing transition from innate to acquired immunity.

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