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Validation Data Gallery

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  • Western Blot (WB) analysis of human peripheral blood platelets using IL-5RA Polyclonal antibody (12655-1-AP)

    WB analysis of human peripheral blood platelets using 12655-1-AP (same clone as 12655-1-PBS)

    human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 12655-1-AP (IL-5RA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12655-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using IL-5RA Polyclonal antibody (12655-1-AP)

    WB analysis of Jurkat using 12655-1-AP (same clone as 12655-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 12655-1-AP (IL5RA antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12655-1-PBS in a different storage buffer formulation.

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IL-5RA Polyclonal antibody, PBS Only

IL-5RA Polyclonal Antibody for WB, Indirect ELISA
Cat No. 12655-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

IL5RA, IL 5RA, IL 5R subunit alpha, IL 5R alpha, IL 5 receptor subunit alpha

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12655-1-PBS targets IL-5RA in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IL-5RA fusion protein Ag3358 相同性解析による交差性が予測される生物種
Full Name interleukin 5 receptor, alpha
Calculated molecular weight 48 kDa, 38 kDa
Observed molecular weight 70-75 kDa, 43 kDa
GenBank accession numberBC027599
Gene Symbol IL-5RA
Gene ID (NCBI) 3568
RRIDAB_2127342
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ01344
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Interleukin-5 receptor subunit alpha (IL-5RA), also known as CD125, is a type I transmembrane protein that belongs to the type I cytokine receptor family. IL-5RA is a critical component of the high-affinity IL-5 receptor complex, IL-5R, which consists of the alpha chain (IL-5RA) that specifically binds to IL-5 and the common beta chain (CD131) shared with receptors for IL-3 and GM-CSF (PMID: 1833065; 7872157). IL-5/IL-5RA signaling axis is crucial for the differentiation, proliferation, recruitment, and activation of eosinophils, and is also involved in pathological eosinophilia (PMID: 34638583).