Validation Data Gallery
Tested Applications
| Positive WB detected in | Recombinant protein |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
30416-1-AP targets IL-5 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Eg0380 Product name: Recombinant Human IL-5 protein (His Tag) Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS Tag: C-6*HIS Domain: 20-134 aa of BC066282 Sequence: IPTEIPTSALVKETLALLSTHRTLLIANETLRIPVPVHKNHQLCTEEIFQGIGTLESQTVQGGTVERLFKNLSLIKKYIDGQKKKCGEERRRVNQFLDYLQEFLGVMNTEWIIES 相同性解析による交差性が予測される生物種 |
| Full Name | interleukin 5 (colony-stimulating factor, eosinophil) |
| Calculated molecular weight | 134 aa, 15 kDa |
| GenBank accession number | BC066282 |
| Gene Symbol | IL5 |
| Gene ID (NCBI) | 3567 |
| RRID | AB_3742341 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P05113 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Interleukin-5 (IL-5) is a pleiotropic cytokine primarily produced by activated T helper 2 (Th2) cells, mast cells, and group 2 innate lymphoid cells (ILC2s). It plays a critical role in the regulation of eosinophil proliferation, differentiation, and activation, making it a key mediator in allergic inflammation, parasitic infections, and certain autoimmune diseases.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for IL-5 antibody 30416-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |