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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IL-5 Polyclonal antibody (30198-1-AP)

    WB analysis using 30198-1-AP

    Untreated, PMA, Ionomycin and Brefeldin A treated EL-4 cells were subjected to SDS PAGE followed by western blot with 30198-1-AP (IL-5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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IL-5 Polyclonal antibody

IL-5 Polyclonal Antibody for WB, ELISA
Cat No. 30198-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, ELISA

Synonyms

Il5, interleukin-5, interleukin 5, Il 5

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inPMA, Ionomycin and Brefeldin A treated EL-4 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30198-1-AP targets IL-5 in WB, ELISA applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name interleukin 5
Calculated molecular weight 15 aa
Observed molecular weight18-20 kDa
GenBank accession numberNM_010558
Gene Symbol Il5
Gene ID (NCBI) 16191
RRIDAB_3669705
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP04401
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Interleukin-5 (IL-5) is a critical cytokine that regulates the growth, activation, and survival of eosinophils. IL-5 is produced by both hematopoietic and non-hematopoietic cells including T cells, granulocytes, and natural helper cells. IL-5 exerts its effects for proliferation and differentiation via receptors that comprise an IL-5-specific α and common β-subunit. Overexpression of IL-5 in vivo significantly increases eosinophils and B cells in number, while mice lacking a functional gene for IL-5 or IL-5 receptor display a number of developmental and functional impairments in B cells and eosinophil lineages.

Protocols

Product Specific Protocols
WB protocol for IL-5 antibody 30198-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols