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Validation Data Gallery

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  • Flow cytometry (FC) experiment of human peripheral blood leukocytes using Anti-Human IL-3RB/CD131 Rabbit Recombinant Antibod (98336-2-RR)

    FC experiment of human peripheral blood leukocytes using 98336-2-RR

    1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human IL-3RB/CD131 Rabbit RecAb (98336-2-RR, Clone: 241823B12) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • Flow cytometry (FC) experiment of human peripheral blood leukocytes using Anti-Human IL-3RB/CD131 Rabbit Recombinant Antibod (98336-2-RR)

    FC experiment of human peripheral blood leukocytes using 98336-2-RR

    1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human IL-3RB/CD131 Rabbit RecAb (98336-2-RR, Clone: 241823B12) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • Flow cytometry (FC) experiment of Transfected HEK-293T cells using Anti-Human IL-3RB/CD131 Rabbit Recombinant Antibod (98336-2-RR)

    FC experiment of Transfected HEK-293T using 98336-2-RR

    1x10^6 HEK-293T cells (left) or IL-3RB/CD131-transfected HEK-293T cells (right) were surface stained with 0.25 ug Anti-Human IL-3 R Beta/CD131 Rabbit RecAb (98336-2-RR, Clone:241823B12) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

  • Flow cytometry (FC) experiment of Transfected HEK-293T cells using Anti-Human IL-3RB/CD131 Rabbit Recombinant Antibod (98336-2-RR)

    FC experiment of Transfected HEK-293T using 98336-2-RR

    1x10^6 HEK-293T cells(left) or IL-3RB/CD131-transfected HEK-293T cells(right) were surface stained with 0.25 ug Anti-Human IL-3 R Beta/CD131 Rabbit RecAb (98336-2-RR, Clone:241823B12)(red) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

Anti-Human IL-3RB/CD131 Rabbit Recombinant Antibody

IL-3RB/CD131 Uni-rAb® Recombinant Antibody for FC
Cat No. 98336-2-RR
Clone No.241823B12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

CSF2RB, CD131, CDw131, GM-CSF/IL-3/IL-5 receptor common beta subunit, IL3RB

Formulation:  PBS, Azide
PBS, Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
PE
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive FC detected inhuman peripheral blood leukocytes, Transfected HEK-293T cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98336-2-RR targets IL-3RB/CD131 in FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Eg1662

Product name: Recombinant Human IL-3RB/CD131 protein (rFc Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 17-443 aa of NM_000395.3

Sequence: WERSLAGAEETIPLQTLRCYNDYTSHITCRWADTQDAQRLVNVTLIRRVNEDLLEPVSCDLSDDMPWSACPHPRCVPRRCVIPCQSFVVTDVDYFSFQPDRPLGTRLTVTLTQHVQPPEPRDLQISTDQDHFLLTWSVALGSPQSHWLSPGDLEFEVVYKRLQDSWEDAAILLSNTSQATLGPEHLMPSSTYVARVRTRLAPGSRLSGRPSKWSPEVCWDSQPGDEAQPQNLECFFDGAAVLSCSWEVRKEVASSVSFGLFYKPSPDAGEEECSPVLREGLGSLHTRHHCQIPVPDPATHGQYIVSVQPRRAEKHIKSSVNIQMAPPSLNVTKDGDSYSLRWETMKMRYEHIDHTFEIQYRKDTATWKDSKTETLQNAHSMALPALEPSTRYWARVRVRTSRTGYNGIWSEWSEARSWDTESVLPMW

相同性解析による交差性が予測される生物種
Full Name colony stimulating factor 2 receptor, beta, low-affinity (granulocyte-macrophage)
Calculated molecular weight97kDa
GenBank accession numberNM_000395.3
Gene Symbol CSF2RB
Gene ID (NCBI) 1439
RRIDAB_3745986
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP32927-1
Storage Buffer PBS with 0.09% sodium azide{{ptg:BufferTemp}}7.3
Storage ConditionsStore at 2 - 8°C. Stable for one year after shipment.

Background Information

IL-3RB/CD131, also known as CSF2RB, is the common subunit of the type I cytokine receptors for granulocyte-macrophage colony-stimulating factor (GM-CSF), interleukin (IL)-3 and IL-5. CD131 is expressed on various immune cells, including hematopoietic progenitor cells, granulocytes, monocytes, macrophages, eosinophils, and mast cells, which plays a crucial role in mediating the effects of cytokines (PMID: 18412971). Tregs of healthy donors constitutively transcribe CSF2RB, but protein expression was much higher after TCR stimulation (PMID: 36532080, 3557440).

Protocols

Product Specific Protocols
FC protocol for IL-3RB/CD131 antibody 98336-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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