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Validation Data Gallery

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  • Flow cytometry (FC) experiment of Transfected HEK-293T cells using Anti-Human IL-3RB/CD131 Rabbit Recombinant Antibod (98336-2-RR)

    FC experiment of Transfected HEK-293T using 98336-2-RR

    1x10^6 HEK-293T cells (left) or IL-3RB/CD131-transfected HEK-293T cells (right) were surface stained with 0.25 ug Anti-Human IL-3 R Beta/CD131 Rabbit RecAb (98336-2-RR, Clone:241823B12) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

  • Flow cytometry (FC) experiment of Transfected HEK-293T cells using Anti-Human IL-3RB/CD131 Rabbit Recombinant Antibod (98336-2-RR)

    FC experiment of Transfected HEK-293T using 98336-2-RR

    1x10^6 HEK-293T cells(left) or IL-3RB/CD131-transfected HEK-293T cells(right) were surface stained with 0.25 ug Anti-Human IL-3 R Beta/CD131 Rabbit RecAb (98336-2-RR, Clone:241823B12)(red) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

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Anti-Human IL-3RB/CD131 Rabbit Recombinant Antibody

IL-3RB/CD131 Uni-rAbTM Recombinant Antibody for FC
Cat No. 98336-2-RR
Clone No.241823B12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

CSF2RB, CD131, CDw131, GM-CSF/IL-3/IL-5 receptor common beta subunit, IL3RB

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive FC detected inTransfected HEK-293T cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in 100 μl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98336-2-RR targets IL-3RB/CD131 in FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen IL-3RB/CD131 fusion protein Eg1662 相同性解析による交差性が予測される生物種
Full Name colony stimulating factor 2 receptor, beta, low-affinity (granulocyte-macrophage)
Calculated molecular weight97kDa
GenBank accession numberNM_000395.3
Gene Symbol CSF2RB
Gene ID (NCBI) 1439
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP32927-1
Storage Buffer PBS with 0.09% sodium azide{{ptg:BufferTemp}}7.3
Storage ConditionsStore at 2 - 8°C. Stable for one year after shipment.

Background Information

IL-3RB/CD131, also known as CSF2RB, is the common subunit of the type I cytokine receptors for granulocyte-macrophage colony-stimulating factor (GM-CSF), interleukin (IL)-3 and IL-5. CD131 is expressed on various immune cells, including hematopoietic progenitor cells, granulocytes, monocytes, macrophages, eosinophils, and mast cells, which plays a crucial role in mediating the effects of cytokines (PMID: 18412971). Tregs of healthy donors constitutively transcribe CSF2RB, but protein expression was much higher after TCR stimulation (PMID: 36532080, 3557440).

Protocols

Product Specific Protocols
FC protocol for IL-3RB/CD131 antibody 98336-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols